3.1.1: Making Media (LB Broth And Agar) - Biology LibreTexts

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LB Agar:

Making 1 L of LB Agar:

  • 5 g Yeast extract
  • 10 g Peptone
  • 10 g NaCl
  • 12 g Agar
  1. Mix this with 1 L of Distilled H2O
  2. Autoclave at 121 °C for 30 minutes (If there is a setting, set it to slow/liquid)
  3. Let cool before pouring into plates (approximately 25 mL of agar in each plate)

This recipe makes about 40 plates and can be adjusted as needed!

LB Broth:

Making 1 L of LB Broth:

  • 25 g pre-mixed broth powder

*This powder contains 10 g tryptone, 5 g yeast extract and 10 g NaCl*

  1. Mix this with 1 L of Distilled H2O
  2. Autoclave at 121 °C for 30 minutes (If there is a setting, set it to slow/liquid)
  3. Let cool before pouring into STERILE test tubes with lids (approximately 5 mL of broth per test tube)

This recipe makes about 200 tubes of broth and can be adjusted as needed!

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