8.3 Cellular Respiration – Microbiology: Canadian Edition

The electron transport system (ETS) comprises a series of membrane-associated protein complexes and associated mobile accessory electron carriers. In bacteria and archaea, this is in the plasma membrane; in eukaryotes, this is in the mitochondrion (phototrophic electron transport systems are very similar, and are discussed later in this chapter). Electron transport is a series of chemical reactions that resembles a bucket brigade in that electrons are passed rapidly from one ETS electron carrier to the next. In heterotrophs, electrons enter the system through the reduced NADH and FADH2 cofactors from the central pathways, or an external or intracellular inorganic energy source in the lithotrophs.

While there are many different electron carriers, some unique to specific organisms or groups of organisms, some of the more common ones are listed below and also in Figure 8.19. Whether a particular ETS component accepts electrons and protons, or just electrons is critical to chemiosmosis and energy generation, as discussed further below.

• Nicotinamide adenine dinucleotide (NAD+/NADH) – a co-enzyme that carriers both electrons (e–) and protons (H+), two of each. A closely related molecule is nicotinamide adenine dinucleotide phosphate (NADP+/ NADPH), which accepts 2 electrons and 1 proton.
• Flavin adenine dinucleotide (FAD/FADH2) and flavin mononucleotide (FMN/FMNH2) – carry 2 electrons and 2 protons each. Proteins with these molecules are called flavoproteins.
• Coenzyme Q (CoQ)/ubiquinone – a small, lipid soluble ETS component that alternates from quinone to quinol upon reduction by 2 electrons and 2 protons.
• Cytochromes – immobile ETS components that use iron atoms as part of a heme group, carrying 1 electron at a time with reduction of ferric iron (Fe3+) to ferrous iron (Fe2+).
• Iron-sulphur (Fe-S) proteins, such as ferredoxin – immobile ETS components that use iron atoms not part of heme group to carry 1 electron at a time.

ETS carriers are arranged so that electrons move spontaneously, based on their redox potential, from those with more negative redox potential (i.e. higher in the tower) to those with more positive redox potential.

Chemiosmosis, Proton Motive Force, and Oxidative Phosphorylation

In each transfer of an electron through the ETS, the electron loses energy, but with some transfers, the energy is stored as potential energy by using it to pump hydrogen ions (H+) across a membrane. In prokaryotic cells, H+ is pumped to the outside of the cytoplasmic membrane (i.e. the periplasmic space in gram-negative and gram-positive bacteria), and in eukaryotic cells, they are pumped from the mitochondrial matrix across the inner mitochondrial membrane into the intermembrane space. There is an uneven distribution of H+ across the membrane that establishes an electrochemical gradient because of the greater density of positively charged H+ ions (ΔΨ) and the higher chemical concentration (i.e.ΔpH) on one side of the membrane. This electrochemical gradient is the proton motive force (Δp or PMF). Beyond the use of the PMF to make ATP, as discussed in this chapter, the PMF can also be used to drive other energetically unfavourable processes, including nutrient transport and flagellar rotation for motility.

The energy in the proton motive force is converted to ATP through a process called chemiosmosis. The protons diffuse across the membrane (the plasma membrane in prokaryotic cells and the inner membrane in mitochondria in eukaryotic cells), following their concentration gradient, through the membrane-bound ATP synthase complex (Figure 8.20). The tendency for movement in this way is much like water accumulated on one side of a dam, moving through the dam when opened. ATP synthase (like a combination of the intake and generator of a hydroelectric dam) is a complex protein that acts as a tiny generator, turning by the force of the H+ diffusing through the enzyme, down their electrochemical gradient from where there are many mutually repelling H+ to where there are fewer H+. In prokaryotic cells, H+ flows from the outside of the cytoplasmic membrane into the cytoplasm, whereas in eukaryotic mitochondria, H+ flows from the intermembrane space to the mitochondrial matrix. The turning of the parts of this molecular machine regenerates ATP from ADP and inorganic phosphate (Pi) by oxidative phosphorylation, a second mechanism for making ATP that harvests the potential energy stored within an electrochemical gradient.

Aerobic Respiration

In aerobic respiration, the final electron acceptor (i.e., the one having the highest or most positive redox potential) at the end of the ETS is an oxygen molecule (O2). This is reduced to water (H2O) by the final ETS carrier complex, which includes a terminal oxidase and usually, a cytochrome. The terminal oxidase differs between bacterial types and can be used to differentiate closely related bacteria for diagnoses. For example, the gram-negative opportunist Pseudomonas aeruginosa and the gram-negative cholera-causing Vibrio cholerae use cytochrome c oxidase, which can be detected using the oxidase test, whereas other gram-negative bacteria like E. coli and other members of the Enterobacteriaceae, are negative for this test because they produce different cytochrome oxidase types.

There are many circumstances under which aerobic respiration is not possible, including any one or more of the following:

• The cell lacks genes encoding an appropriate cytochrome oxidase for transferring electrons to oxygen at the end of the electron transport system.
• The cell lacks genes encoding enzymes to minimize the severely damaging effects of dangerous oxygen radicals produced during aerobic respiration, such as hydrogen peroxide (H2O2) or superoxide (O2–).
• The cell lacks a sufficient amount of oxygen to carry out aerobic respiration.

Anaerobic Respiration

One possible alternative to aerobic respiration is anaerobic respiration, using an inorganic molecule other than oxygen as a final electron acceptor. There are many types of anaerobic respiration found in bacteria and archaea. Denitrifiers are important soil bacteria that use nitrate (NO3–) and nitrite (NO2–) as final electron acceptors, producing nitrogen gas (N2). Many aerobically respiring bacteria, including E. coli, switch to using nitrate as a final electron acceptor and producing nitrite when oxygen levels have been depleted.

Microbes using anaerobic respiration commonly have an intact Krebs cycle, so these organisms can access the energy of the NADH and FADH2 molecules formed. However, anaerobic respirers use altered ETS carriers encoded by their genomes, including distinct complexes for electron transfer to their final electron acceptors. Smaller electrochemical gradients are generated from these electron transfer systems, so less ATP is formed through anaerobic respiration. The energy yield differences are explained by the differences in . As depicted in Figure 8.19, reduction of oxygen to water is at the bottom of the redox tower while alternate terminal electron acceptors are higher up the tower.

Denitrification

Denitrification is the utilization of nitrate (NO3−) as the terminal electron acceptor. Nitrate, like oxygen, has a relatively positive reduction potential. This process is widespread, and used by many members of the Proteobacteria. Many denitrifying bacteria can also use ferric iron (Fe3+) and different organic electron acceptors. Denitrification involves the stepwise reduction of nitrate to nitrite (NO2–), nitric oxide (NO), nitrous oxide (N2O), and, eventually, to dinitrogen (N2).

Some organisms (e.g. E. coli) only produce nitrate reductase, the enzyme that catalyzes the first reduction leading to the accumulation of nitrite. Others (e.g. Paracoccus denitrificans or Pseudomonas stutzeri) reduce nitrate completely. Complete denitrification is an environmentally significant process because nitrous oxide is a powerful greenhouse gas, while nitric oxide reacts with sunlight and ozone to produce nitric acid, a component of acid rain. Denitrification is also important in biological wastewater treatment, where it can be used to reduce the amount of nitrogen released into the environment, thereby reducing eutrophication.

Denitrification takes place under special conditions in both terrestrial and marine ecosystems, where oxygen consumption exceeds the oxygen supply and sufficient quantities of nitrate are present. These environments may include certain soils and groundwater, wetlands, oil reservoirs, and in sea floor sediments.

Sulphate and Sulphur Reduction

Sulfate reduction uses sulphate (SO42-) as the electron acceptor, producing hydrogen sulphide (H2S) as a metabolic end product. Sulfate reduction is a relatively energetically poor process, though it is a vital mechanism for bacteria and archaea living in oxygen-depleted, sulphate-rich environments. Many sulphate-reducing organisms are organotrophic, using carbon compounds, such as lactate and pyruvate (among many others) as electron donors. Other sulphate reducers are lithotrophic, using hydrogen gas (H2) as an electron donor. Some species of sulphate-reducing bacteria (SRBs; e.g., Desulfovibrio sulfodismutans, Desulfocapsa thiozymogenes, and Desulfocapsa sulfoexigens) are capable of sulphur disproportionation (splitting one compound into two different compounds, in this case an electron donor and an electron acceptor) using elemental sulphur (S0), sulphite (SO32−), and thiosulphate (S2O32−) to produce both hydrogen sulphide (H2S) and sulphate (SO42−).

Sulfate-reducing bacteria are common in anaerobic environments (such as seawater, sediment, and water rich in decaying organic material) where they aid in the degradation of organic materials. In these anaerobic environments, fermenting bacteria extract energy from large organic molecules; the resulting smaller compounds (such as organic acids and alcohols) are further oxidized by acetogens, methanogens, and the competing SRBs. Acetogens and methanogens are further discussion in Methanogens and Syntrophy.

Many bacteria reduce small amounts of sulphates in order to synthesize sulphur-containing cell components; this is known as assimilatory sulphate reduction. By contrast, sulphate-reducing bacteria reduce sulphate in large amounts to obtain energy and expel the resulting sulphide as waste; this is known as “dissimilatory sulphate reduction”. Most SRBs can also reduce other oxidized inorganic sulphur compounds, such as sulphite, thiosulphate, or elemental sulphur (S0).

Toxic hydrogen sulphide is one waste product of sulphate-reducing bacteria; its rotten egg odour is often a marker for the presence of SRBs. Common examples are salt marshes and mud flats. Much of the hydrogen sulphide will react with metal ions in the water to produce metal sulphides. These metal sulphides, such as ferrous sulphide (FeS), are insoluble and often black or brown. Thus, the black colour of sludge on a pond, or in the Winogradsky sulphuretum (Figure 8.21), is due to metal sulphides that result from the action of sulphate-reducing bacteria.

Other Alternative Electron Acceptors

Ferric iron (Fe3+) is a widespread anaerobic terminal electron acceptor used by both autotrophic and heterotrophic organisms. Electron flow in these organisms is similar to those in electron transport, ending in oxygen or nitrate, except that in ferric iron-reducing organisms the final enzyme in this system is a ferric iron reductase. Since some ferric iron-reducing bacteria (e.g. Geobacter metallireducens) can use toxic hydrocarbons (e.g. toluene) as a carbon source, there is significant interest in using these organisms as bioremediation agents in ferric iron contaminated aquifers.

Other examples of anaerobic respiration include the reduction of Manganic ion (Mn4+) to manganous (Mn2+), Selenate (SeO42−) to selenite (SeO32−) to selenium (Se), Arsenate (AsO43−) to arsenite (AsO33-), and Uranyl (UO22+) to uranium dioxide (UO2)

Organic compounds may also be used as electron acceptors in anaerobic respiration. These include the reduction of fumarate to succinate and dimethyl sulfoxide (DMSO) to dimethyl sulphide (DMS).

Methanogenesis is another form of anaerobic respiration involving the reduction of CO2. It is discussed in Methanogens and Syntrophy.

• What are the functions of the proton motive force?
• What are examples of different electron acceptors in anaerobic respiration?