Blood Freezing To Nearly Absolute Zero Temperature: -272.29 Degrees C

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Abstract

Human erythrocytes were collected in ACD anticoagulant, glycerolized with an equal volume of 8.6 M glycerol, 8 percent glucose, 1 percent fructose, and 0.3 percent Na2 EDTA solution, sealed in glass ampules, and frozen to -272.29 degrees C (0.86 degrees K) in liquid helium under high vacuum. Biological assays performed after thawing and three-stage dialysis to remove the glycerol showed less than 1 percent hemolysis. Intracellular potassium, 2,3 DPG, and ATP were within the normal range.

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