Mechanism of thioflavin T binding to amyloid fibrils - PubMed pubmed.ncbi.nlm.nih.gov › ...
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22 Apr 2010 · ThT binds to diverse fibrils, despite their distinct amino acid sequences, strongly suggesting that ThT recognizes a structural feature common ... Abstract · Introduction · Atomic level studies of ThT... · Conclusions and future...
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1 Jan 2017 · Thioflavin T (ThT) is a commonly used probe to monitor in vitro amyloid fibril formation. Upon binding to amyloid fibrils, ThT gives a strong ... Abstract · Introduction · Results and discussion · Material and methods
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Thioflavins are fluorescent dyes that are available as at least two compounds, namely Thioflavin T and Thioflavin S. Both are used for histology staining ...
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When it binds to β-sheet-rich structures such as amyloid fibrils, ThT displays enhanced fluorescence and a characteristic blue shift in the emission spectrum [4 ...
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16 Apr 2015 · ThT, a typical probe for amyloid fibrils, binds to human telomeric G-quadruplex DNA with a fluorescent light-up signal change (24–26). Here, we ...
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19 May 2017 · Thioflavin T (ThT) fluorescence is a proven method to detect the amyloid fibrils that frequently accompany the development of serious human ...
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1B) is, how- ever, clearly affected by quenching of the ThT fluorescence and cannot be attributed solely to an inhibitory effect on fibril formation (compare ...
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28 Oct 2008 · The fluorescent dye thioflavin T (ThT) is commonly used for in situ amyloid fibril detection. In this work, we focused on the spectroscopic ...
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24 Feb 2012 · Benzothiazole dye thioflavin T (ThT) fluorescence is a well-known test for amyloid fibril formation in diseases associated with disturbances in ...
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25 Sept 2009 · Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of ...
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11 Aug 2021 · Currently, fluorescence-based imaging using Thioflavin T (ThT) dye is the gold standard for detecting protein aggregation. It is used to detect ...
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29 Oct 2010 · In this work, the fluorescence of thioflavin T (ThT) was studied in a wide ... as this dye does not interact with proteins in their folded, ...
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Combining X-ray data on thioflavin-T and theoretical calculations on its binding to a peptide model for Aβ1–42 fibrils gives evidence of main stabilizing ...
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Component C Thioflavin T (ThT) ... 1.1 ThT working solution: Add 100 µl of 20mM ThT (Component C) to 900 µl of the ... Do not vortex, mix by inversion only.
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