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Pets and Animals
Dogs
Breeding Dogs
How to Artificially Inseminate a Dog Download ArticleLearn to collect and implant dog semen at home for safe DIY breeding Co-authored by John Jess
Last Updated: June 20, 2025 Fact Checked
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Evaluating Both Dogs
|
Collecting Dog Semen
|
Implanting Semen into the Female
|
Tips
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This article was co-authored by John Jess. John Jess is a Dog Breeder and the Founder of Central Illinois Doodles, a family business located in Arthur, Illinois. He specializes in breeding Goldendoodles and Bernedoodles. Central Illinois Doodles has been named one of the Top 10 Bernedoodle breeders in the United States by Bubbly Pet and Pupvine. They’ve also been named one of the best Goldendoodle breeders by Trending Breeds and Devoted to Dog. There are 9 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 267,612 times.
Artificial insemination (AI) is the technique used to impregnate a female dog without that dog having sex. First semen is collected from a male dog and then it is placed into a female dog’s reproductive tract while she is fertile. This, hopefully, will result in the sperm penetrating the female's egg, and producing an embryo. While it is a technique that is performed by experienced dog breeders, it takes a lot of knowledge, skill, and practice to prefect, and so should only be done by trained professionals.
This article is partially based on an interview with John Jess, a Dog Breeder and the Founder of Central Illinois Doodles, a family business located in Arthur, Illinois.
Things You Should Know
Evaluate both dogs to confirm they're healthy enough to breed.
Bring the male near the female when she's in heat. When he goes to mount her, redirect his penis into an artificial vagina to catch the semen.
Chill or freeze the semen if you aren't using it immediately for insemination.
Insert the semen into the female dog's vagina with a pipette when she's in the correct stage of her estrus cycle.
Steps
Part 1 Part 1 of 3:
Evaluating Both Dogs
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1 Determine whether or not to use artificial insemination. Dog breeders use artificial insemination for a variety of reasons. Some breeds suffer from a diminished gene pool, especially if the country has a small number of breeding dogs.[1]XResearch source If the male and female dog are separated by great distances it may be cheaper to AI than to pay for transportation for the dog and their handler.
Artificial insemination can also make the semen from a desirable male dog more widely available.[2]X Expert Source John JessDog BreederExpert Interview
2 Evaluate the male dog for suitability for breeding. Only dogs that are healthy and meet breed specifications for conformation and type should be bred. This helps assure that the offspring will be healthy and display the desired breed traits.[3]XResearch source
In addition to a general health examination, a breeding soundness examination is done to make sure the male dog’s reproductive tract is healthy and sound.
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3 Evaluate the female dog for suitability for breeding. Much like the male, the female dog needs to be examined to make sure she is healthy for breeding and that she meets breed standards. A female dog that is in ill health or who has reproductive problems could have problems getting pregnant, even with AI, or could suffer severe health problems due to a pregnancy.[4]XResearch source
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Collecting Dog Semen
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1 Collect the male dog's semen. Once the male dog has been deemed acceptable for breeding his semen will need to be collected. Male dogs will become aroused in the presence of a female dog in heat, which is the prime time her body emits pheromones or chemicals that excite the males. When he attempts to mount, or breed, with the female the penis is redirected into an artificial vagina into which he ejaculates.[5]XResearch source
In some cases a female at the correct stage of heat is not available. Frozen cotton swabs that were wiped in the vagina of a female dog at the peak of heat can be thawed and swiped on the tail end of any dog, which causes the male dog to respond.
In some cases semen can be collected and stored before a dog dies to be made available after his death; in others injury may make it impossible for a male dog to breed naturally yet he may still be able to produce semen.
2 Have the semen evaluated. After the semen is collected, the sperm cells in it will be examined under a microscope to make sure they are normal. This means that there will be sufficient numbers, shape, and activity.[6]XResearch source
3 Chill or freeze semen that it not used immediately. Chilled semen is used within 24 hours or else it will need to be frozen. Semen is stored in liquid nitrogen containers at subzero temperatures. This way semen can be used years later.[7]X Trustworthy Source American Kennel ClubThe American Kennel Club (AKC) is a purebred dog pedigree registry in the United States. The AKC advocates for the responsible ownership of dogs and promotes purebred dog events, such as the Westminster Dog Show. Go to source
The best success rate comes from use of fresh semen. With chilled semen the success rate drops to 59-80% and with frozen it falls to 52-60%.
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Implanting Semen into the Female
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1 Make sure the female dog is in the correct stage of her estrus cycle. Unlike the male, she needs to be in the receptive stage of her heat cycle in order to breed successfully. Females typically come into heat twice a year.[8]XResearch source
Each heat cycle has a preparatory proestrus stage, where the female’s vulva (visible to the outside) swells and she bleeds or spots blood for seven to nine days. The uterus (womb) is being prepared to make a suitable environment for the embryos to develop into puppies.[9]XResearch source
After this, the bleeding slows and she becomes receptive to breeding. This stage is called estrus. This is also called standing heat, because it is the period when the female will stand to mated with the male dog. It is only during this stage that she will be able to become pregnant. The most fertile time will be at the end or just after standing heat occurs.
Veterinarians can determine the stage of heat the female dog is in by collecting swabs inside the vagina and examining the cells.[10]XResearch source The swabs will collect cells which have distinctive shapes and sizes depending on the stage of estrus. This is especially helpful in those female dogs that don’t swell or bleed much.
The best time to inseminate is going to be at peak ovulation, which is determined based on the progesterone numbers. Monitor the levels with your vet and wait from 48 to 72 hours after the spike in progesterone to inseminate.[11]X Expert Source John JessDog BreederExpert Interview
2 Insert the semen into the female dog's vagina. Insemination is fairly straight forward once the semen is collected and the female dog is in estrus. The female is stood on the examination table with her back legs slightly extended beyond the pelvis. A pipette is used to deposit the semen inside the vagina near the cervix.[12]XResearch source
The insertion of the pipette takes special skill as the female dog’s vagina is quite long. The pipette is withdrawn and discarded.
3 Keep the dog standing after the semen is inserted. She should be kept standing for 10 minutes after depositing the semen. She should not sit or urinate, or the semen may run out.
After 10 minutes she can walk around normally, but jumping and urinating should be avoided for about an hour.
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Expert Q&A
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Tips
Ask your veterinarian for advice on timing your particular dog’s breeding. Thanks Helpful 1 Not Helpful 0
Read all that you are able to on breed standards for your particular breed to help you make the best choice for the breeding pair. Thanks Helpful 0 Not Helpful 0
If you have no experience with dog breeding, hire a professional to help you with the process. Thanks Helpful 0 Not Helpful 0
Show More Tips Submit a Tip All tip submissions are carefully reviewed before being published Name Please provide your name and last initial Submit Thanks for submitting a tip for review! Advertisement
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Co-authored by: John Jess Dog Breeder This article was co-authored by John Jess. John Jess is a Dog Breeder and the Founder of Central Illinois Doodles, a family business located in Arthur, Illinois. He specializes in breeding Goldendoodles and Bernedoodles. Central Illinois Doodles has been named one of the Top 10 Bernedoodle breeders in the United States by Bubbly Pet and Pupvine. They’ve also been named one of the best Goldendoodle breeders by Trending Breeds and Devoted to Dog. This article has been viewed 267,612 times. 33 votes - 77% Co-authors: 8Updated: June 20, 2025 Views: 267,612 Categories: Breeding DogsArticle SummaryX
Before artificially inseminating your dog, take it to your vet to make sure it’s in good health. If so, the vet will collect the semen and examine it to make sure the sperm cells look normal. If you don't plan on using the semen right away, ask your vet to chill or freeze it. Once the female dog is ready, the vet will use a pipette to insert the semen in to the uterus. For more advice from our Veterinary reviewer, including how to care for your dog after insemination, keep reading.Did this summary help you?YesNo
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Reader Success Stories
Enoch Achadu
Jun 26, 2021
"I had issues concerning my bitch who refuses to breed but now I have gotten the solution for a successful breeding."
More reader stories Hide reader stories Share your story
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Yes No Advertisement Cookies make wikiHow better. By continuing to use our site, you agree to our cookie policy. Co-authored by: John Jess Dog Breeder 33 votes - 77% Click a star to vote Co-authors: 8Updated: June 20, 2025 Views: 267,612
Enoch Achadu
Jun 26, 2021
"I had issues concerning my bitch who refuses to breed but now I have gotten the solution for a successful breeding."
Ed C.
Apr 21, 2025
"It is great information to have on AI." Share yours! More success stories Hide success stories
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Download Article Learn to collect and implant dog semen at home for safe DIY breeding Co-authored by John Jess 
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Co-authored by: John Jess Dog Breeder This article was co-authored by John Jess. John Jess is a Dog Breeder and the Founder of Central Illinois Doodles, a family business located in Arthur, Illinois. He specializes in breeding Goldendoodles and Bernedoodles. Central Illinois Doodles has been named one of the Top 10 Bernedoodle breeders in the United States by Bubbly Pet and Pupvine. They’ve also been named one of the best Goldendoodle breeders by Trending Breeds and Devoted to Dog. This article has been viewed 267,612 times. 33 votes - 77% Co-authors: 8 Updated: June 20, 2025 Views: 267,612 Categories: Breeding Dogs Article SummaryX
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1 Determine whether or not to use artificial insemination. Dog breeders use artificial insemination for a variety of reasons. Some breeds suffer from a diminished gene pool, especially if the country has a small number of breeding dogs.[1] If the male and female dog are separated by great distances it may be cheaper to AI than to pay for transportation for the dog and their handler. 
2 Evaluate the male dog for suitability for breeding. Only dogs that are healthy and meet breed specifications for conformation and type should be bred. This helps assure that the offspring will be healthy and display the desired breed traits.[3] 
3 Evaluate the female dog for suitability for breeding. Much like the male, the female dog needs to be examined to make sure she is healthy for breeding and that she meets breed standards. A female dog that is in ill health or who has reproductive problems could have problems getting pregnant, even with AI, or could suffer severe health problems due to a pregnancy.[4] Advertisement 
1 Collect the male dog's semen. Once the male dog has been deemed acceptable for breeding his semen will need to be collected. Male dogs will become aroused in the presence of a female dog in heat, which is the prime time her body emits pheromones or chemicals that excite the males. When he attempts to mount, or breed, with the female the penis is redirected into an artificial vagina into which he ejaculates.[5] 
2 Have the semen evaluated. After the semen is collected, the sperm cells in it will be examined under a microscope to make sure they are normal. This means that there will be sufficient numbers, shape, and activity.[6] 
3 Chill or freeze semen that it not used immediately. Chilled semen is used within 24 hours or else it will need to be frozen. Semen is stored in liquid nitrogen containers at subzero temperatures. This way semen can be used years later.[7] 
1 Make sure the female dog is in the correct stage of her estrus cycle. Unlike the male, she needs to be in the receptive stage of her heat cycle in order to breed successfully. Females typically come into heat twice a year.[8] 
2 Insert the semen into the female dog's vagina. Insemination is fairly straight forward once the semen is collected and the female dog is in estrus. The female is stood on the examination table with her back legs slightly extended beyond the pelvis. A pipette is used to deposit the semen inside the vagina near the cervix.[12] 
3 Keep the dog standing after the semen is inserted. She should be kept standing for 10 minutes after depositing the semen. She should not sit or urinate, or the semen may run out. 