Measure Area Fraction - Forum Home » Area Fraction Imagej » Measure Area Fraction - Forum Maybe your like Area Gestion Sae Curriculum Area If Triangle Formula Area J Elementary School Area K2 Hampden Are Ak47 And Ak74 Parts Interchangeable Measure area fraction Image Analysis segmentation zgmei (Zhigang Mei) March 18, 2020, 3:44am 1 Hi, I am a new user of ImageJ/FIJI. I am trying to measure the area fractions of the gold, grey and white region in the attached figure using ImageJ/FIJI. The gold regions have distinct edges and are relatively easy to separate out. The grey and white regions are much harder for me to separate. Please let me know how I can separate the grey and white regions and measure their area fraction. Thanks. image1670×1274 2.66 MB Mathew March 18, 2020, 5:28am 2 Hi Mei @zgmei Something similar was dealt with there: Distinguishing between two types of soil For your image I suggest: RGB-> Edit->Invert->RGB to CMYK-> Stack to Images-> Thresholding-> Measure Can you tell me about your results, if you don’t mind. Below is a macro to find the white area. Greetings Flatten-fraction-11670×1274 670 KB requires("1.52t"); // -------------------------Clean and preparate environment print("\\Clear"); run("Clear Results"); roiManager("reset") ; if ( isOpen("Summary") ) { run("Close"); } // ---------------------------Macro start setBackgroundColor(0,0,0); setOption("BlackBackground",true); img=getImageID(); selectImage(img); setBatchMode(true); run("Duplicate...", "title=original"); run("Duplicate...", " "); run("Invert"); run("RGB to CMYK"); //selectWindow("CMYK_2"); run("Stack to Images"); selectImage("Y"); run("Duplicate...", "title=Y-1"); rename("Whitezone"); setAutoThreshold("Default dark"); //run("Threshold..."); //setOption("BlackBackground", true); run("Convert to Mask"); run("Set Measurements...", "area redirect=None decimal=2"); run("Analyze Particles...", "display summarize add"); roiManager("Show All without labels"); roiManager("Set Fill Color", "blue"); selectWindow("Whitezone"); roiManager("Set Fill Color", "blue"); run("Flatten"); setBatchMode(false); if (nImages>0) { showMessage("Warning", "All images will be closed now!"); close("*"); } // ---------------------------------------------- Macro end selectWindow("Summary"); setLocation(0,0); exit("For whitezone, all is done!"); zgmei (Zhigang Mei) March 19, 2020, 4:06pm 3 Hi Mathew, Thank you so much for your reply and macro. The figure you posted is exactly what I wanted. Some improvements could be to exclude the small blue dots in grey regions. In the original picture, those small white dots in the grey regions are artifacts. Overlaps between different regions should be minimized. Something like the following will be more realistic. Let me know if you can help me further. processed1337×1139 307 KB Thanks, Zhi-Gang 1 Like Related topics Topic Replies Views Activity Counting areas with diferent colors Image Analysis imagej 20 12214 March 31, 2016 Need to quantify area with white spots within a black/colored background Image Analysis analyze-particles 7 1590 November 7, 2019 Single-color area analysis Image Analysis imagej 4 1588 August 8, 2022 Area fraction and dimensions of complex phases Image Analysis imagej 2 485 November 24, 2022 Calculate area fraction of phase in image Image Analysis imagej 14 1911 September 8, 2022 Tag » Area Fraction Imagej Analyze Menu - ImageJ Area Fraction Using ImageJ - YouTube ImageJ - Area Fraction Measurement (Metallography) - YouTube ImageJ Tutorial Area And Threshold - YouTube How To Calculate Area Fraction Of Any Color On Multi-colored Grains In ... (PDF) Phase Fraction Calculation Of Microstructure Using ImageJ Brief Instructions For ImageJ Imagej Tutorial Area And Threshold - Otosection How Is Area Calculated In ImageJ? - High Tech Guide [PDF] MSE360 - Digital Quantitative Metallography - UMich MSE [PDF] FIJI/Image J For Beginners Fundamentals Of Image Quantification Part 4—Measure Areas Using Thresholding - SERC - Carleton ImageJ Analysis - MBW - Michael Hooker Microscopy Facility