Nervous System - Active Transport: The Sodium-potassium Pump

Passive transport: membrane channels

The sodium-potassium pump sets the membrane potential of the neuron by keeping the concentrations of Na+ and K+ at constant disequilibrium. The sudden shift from a resting to an active state, when the neuron generates a nerve impulse, is caused by a sudden movement of ions across the membrane—specifically, a flux of Na+ into the cell. Given the relative impermeability of the plasma membrane to Na+, this influx itself implies a sudden change in permeability. Beginning in the 19th century, researchers puzzled over the mechanism by which this change could occur. The idea arose that there must exist pores, or channels, through which the ions could diffuse, passing the barrier posed by the lipid bilayer. However, for years only the gross currents accompanying ionic movement could be measured, and it was only by inference that the presence of membrane channels could be postulated.

The breakthrough came in the 1970s and ’80s with the development of the patch-clamp technique, which enabled researchers to directly measure currents flowing across single ion channels in the membrane. The patch-clamp technique electrically isolates a small patch of neuron or muscle cell membrane by applying the tip of a micropipette filled with conducting solution to the membrane and forming a tight seal with it. As single channels in the patch undergo various transitional states between fully open and fully closed, the times of opening and closing are recorded and the amplitudes and duration of the currents are measured.

Since the pioneering studies, the electrical and biochemical properties of certain channels have been characterized. Known as “voltage dependent” when activated by changes in the membrane potential and “neurotransmitter sensitive” when activated by neurotransmitter substances, these channels are protein structures that span the membrane from the extracellular space to the cytoplasm. They are thought to be cylindrical, with a hollow, water-filled pore wider than the ion passing through it except at one region called the selectivity filter. This filter makes each channel specific to one type of ion.

Sodium channels

Voltage-sensitive sodium channels have been characterized with respect to their subunit structure and their amino acid sequences. The principal protein component is a glycoprotein containing 1,820 amino acids. Four similar transmembrane domains, of about 300 amino acids each, surround a central aqueous pore through which the ions pass. The selectivity filter is a constriction of the channel ringed by negatively charged carbonyl oxygens, which repel anions but attract cations. Also within the channel are thought to be two types of charged particles forming the gates that control the diffusion of Na+. One gate closes at polarization and opens at depolarization; the other closes at depolarization.

It is thought that the resting, activated, and inactivated states of the sodium channel are due to voltage-dependent conformational changes in the glycoprotein component. These changes result from effects of the electrical field on the charges and dipoles of the amino acids within the protein. With a large electrical field applied to it, the protein has been observed to change its conformation from a stable, closed resting state to a stable, open state in which the net charge or the location of the charge on the protein is changed.

Potassium channels

There are several types of voltage-dependent potassium channels, each having its own physiological and pharmacological properties. A single neuron may contain more than one type of potassium channel.

The best-known flow of K+ is the outward current following depolarization of the membrane. This occurs through the delayed rectifier channel (IDR), which, activated by the influx of Na+, counteracts the effect of that cation by allowing the discharge of K+. By repolarizing the membrane in this way, the IDR channel restricts the duration of the nerve impulse and participates in the regulation of repetitive firing of the neuron.

Another outward K+ current, occurring with little delay after depolarization, is the A current. IA channels are opened by depolarization following hyperpolarization. By increasing the interval between action potentials, they help a neuron to fire repetitively at low frequencies.

Another type of potassium channel, the IK(Ca) channel, is activated by high concentrations of intracellular Ca2+. The opening of these channels results in hyperpolarization of the membrane, so that they appear to slow the repetitive firing of nerve impulses.

The IM channel is opened by depolarization but is deactivated only by the neurotransmitter acetylcholine. This property may serve to regulate the sensitivity of neurons to synaptic input.

A final type of potassium channel is the anomalous, or inward, rectifier channel (IIR). This channel closes with depolarization and opens with hyperpolarization. By allowing an unusual inward diffusion of K+, the IIR channel prolongs depolarization of the neuron and helps produce long-lasting nerve impulses.

Calcium channels

As with potassium channels, there is more than one type of calcium channel. The inward calcium current is slower than the sodium current. There are at least two types of current in certain neurons of the central nervous system—a long-lasting current activated at positive potential and a transient current activated at more negative potential. There are two corresponding types of calcium channels: a large conductance channel that gives rise to a long-lasting current at positive membrane potentials and a low conductance channel that gives rise to a transient current at more negative potentials. In some neurons a third channel current occurs that is transient and can only be activated at high negative potential.

Anion channels

There may be channels that pass anions such as Cl−, but their existence is difficult to prove. Single-channel recordings of cultured tissue have shown selective Cl− channels that are voltage dependent and of high conductance. Channels with lower conductance have been demonstrated in reconstituted artificial membranes as well as in neurons.