Periodic Acid-Schiff-alcian Blue: A Method For The Differential Staining ...

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Abstract

The staining mechanism underlying the periodic acid-Schiff (PAS)-Alcian Blue (AB) sequence has been investigated using a variety of glycoprotein-containing tissues from different organs of the monkey, rat and mouse. The results obtained suggest that reactive carbohydrates contain at least three types of chemical end-groups found in neutral and acidic glycoproteins: (1) PA-engendered aldehyde groups coloured magenta by the Schiff reagent; (2) PA-engendered aldehyde groups coloured blue bisulphite-AB; and (3) naturally occurring acidic (carboxyl and/or sulphate) groups coloured blue by AB only. The PAS-AB sequence showed heterogeneity of glycoprotein structures in the conjunctiva and the duodenal goblet cells. Thus, the PAS-AB sequence is not the simple reverse sequence of AB-PAS but has its own definite and unique staining selectivity and can hence be used as a reliable method for the histochemistry of glycoproteins at the light microscope level.

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References

1. 1. J Histochem Cytochem. 1960 Mar;8:135-7 - PubMed
2. 1. Histochem J. 1975 Jan;7(1):85-9 - PubMed
3. 1. Histochemistry. 1984;80(2):129-32 - PubMed
4. 1. J Histochem Cytochem. 1961 Sep;9:553-9 - PubMed
5. 1. Histochemistry. 1975 Jun 9;43(4):333-48 - PubMed

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