Polymyxin B Sulfate - Thermo Fisher

Polymyxin B SulfateCitations & References (2)Gibco™Polymyxin B SulfatePolymyxin B Sulfate is produced by Bacillus polymyxa. Polymyxin B Sulfate works by binding to the cellular membrane and interferingRead moreHave Questions?

Catalog Number	Quantity
21850029	25 MU

Catalog number 21850029Price (USD) - Quantity:25 MUPolymyxin B Sulfate is produced by Bacillus polymyxa. Polymyxin B Sulfate works by binding to the cellular membrane and interfering with its permeability. Gibco™ Polymyxin B Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 100 units/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.Product UseFor Research Use Only: Not intended for animal or human diagnostic or therapeutic use.For Research Use Only. Not for use in diagnostic procedures.SpecificationsConcentration100 Units/mLFor Use With (Application)Bacterial SelectionQuantity25 MUShelf Life24 MonthsShipping ConditionRoom TemperatureFormPowderProduct TypeAntibioticSterilityNon-sterileUnit SizeEachContents & StorageStorage conditions: 15 to 30°CShipping conditions: AmbientShelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. 1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant. 2. Isolate the contaminated culture from other cell lines. 3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters. 4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin. The following is a suggested procedure for determining toxicity levels and decontaminating cultures: 1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage. 2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL. 3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding. 4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration. 5. Culture the cells for one passage in antibiotic-free media. 6. Repeat step 4. 7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated. Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & ReferencesAbstractThe tumour suppressor CDKN2A/p16Authors:Wouters K, Deleye Y, Hannou SA, Vanhoutte J, Maréchal X, Coisne A, Tagzirt M, Derudas B, Bouchaert E, Duhem C, Vallez E, Schalkwijk CG, Pattou F, Montaigne D, Staels B, Paumelle RJournal:Diab Vasc Dis ResPubMed ID:28868898The genomic CDKN2A/B locus, encoding p16Progranulin in the hematopoietic compartment protects mice from atherosclerosis.Authors:Nguyen AD, Nguyen TA, Singh RK, Eberlé D, Zhang J, Abate JP, Robles A, Koliwad S, Huang EJ, Maxfield FR, Walther TC, Farese RVJournal:AtherosclerosisPubMed ID:30212683Progranulin is a circulating protein that modulates inflammation and is found in atherosclerotic lesions. Here we determined whether inflammatory cell-derived progranulin impacts atherosclerosis development.