Retardation Factor | Science - Britannica

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role in chemical separation

• In chemical analysis: Liquid chromatography

  …is performed by comparing the retardation factor (Rf) of the analyte components with the retardation factors of known substances. The retardation factor is defined as the distance from the original sample spot that the component has moved divided by the distance that the mobile phase front has moved and is…

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• In chromatography: Development chromatography

  …reported in terms of the Rf value, the distance moved by the centre of the zone relative to the distance moved by the mobile phase front, where both are measured from the origin. Use of the solvent front as a reference point is frequently inconvenient. A standard solute is often…

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