Temperature Effects | Worthington Biochemical

Home » How Does Temperature Affect Enzyme Activity » Temperature Effects | Worthington Biochemical

Maybe your like

Skip to main content

For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order Caret

Please enter catalog number and quantity

Catalog number Quantity CART Cart

User account menu

  • Log in
Search X

Main navigation

  • Overview
  • Intro to Enzymes
  • Enzyme Manual
  • Tissue Dissociation Guide
  • Resource Library
  • Tissue Tables

Main navigation

  • Products
    • Product Catalog
    • Research Areas
    • Bulk / OEM
    • International Sales
  • Tools & Resources
    • Overview
    • Intro to Enzymes
    • Enzyme Manual
    • Tissue Dissociation Guide
    • Resource Library
    • Tissue Tables
  • Support
    • Overview
    • Customer
    • Technical
    • Collagenase Sampling Program
    • Distributors
  • About Us
    • Overview
    • Company Profile
    • Operations
    • Quality
    • News and Events
    • Privacy & Terms of Use

Introduction to Enzymes

Temperature Effects

Enzyme Figure 13

Figure 13: The effect of temperature on the reaction rate.

Like most chemical reactions, the rate of an enzyme-catalyzed reaction increases as the temperature is raised. A ten degree Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100%. Variations in reaction temperature as small as 1 or 2 degrees may introduce changes of 10 to 20% in the results. In the case of enzymatic reactions, this is complicated by the fact that many enzymes are adversely affected by high temperatures. As shown in Figure 13, the reaction rate increases with temperature to a maximum level, then abruptly declines with further increase of temperature. Because most animal enzymes rapidly become denatured at temperatures above 40°C, most enzyme determinations are carried out somewhat below that temperature.

Over a period of time, enzymes will be deactivated at even moderate temperatures. Storage of enzymes at 5°C or below is generally the most suitable. Some enzymes lose their activity when frozen.

Effects of pH File Introduction to Enzymes (272.27 KB)

Introduction Enzymes

  • Enzymes and Life Processes
    • Early Enzyme Discoveries
    • Chemical Nature of Enzymes
    • Specificity of Enzymes
    • Naming and Classification
  • Enzyme Kinetics
    • Basic Enzyme Reactions
    • Energy Levels
    • The Enzyme Substrate Complex
    • Chemical Equilibrium
    • Factors Affecting Enzyme Activity
  • Additional Resources
    • References
    • Introduction to Enzymes Video

Tag » How Does Temperature Affect Enzyme Activity