Generation and characterization of Arabidopsis T-DNA insertion ... pubmed.ncbi.nlm.nih.gov › ...
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The T-DNA sequence-indexed mutant collections contain insertional mutants for most Arabidopsis thaliana genes and have played an important role in plant ... Summary · Introduction · Materials · Methods
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Insertional mutagenesis is an alternative means of disrupting gene function and is based on the insertion of foreign DNA into the gene of interest. In ... INTRODUCTION · SATURATING THE GENOME... · SCREENING LARGE...
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T-DNA insertion mutagenesis is one of those re- cently developed genetic techniques which can be expected to have a major impact in plant molec-.
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Aug 6, 2021 · The average number of T-DNA insertions per A. thaliana T-DNA insertion line is assumed to be about 1.5 [4]. However, the insertion lines ...
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A: The T-DNA may insert in more than one place within the Arabidopsis genome. Due to the nature of the T-DNA integration event and the limitations of the PCR ...
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T-DNA insertion lines provide important resource for genetic analysis based on mutagenesis in plant research. SALK lines are the most widely used T-DNA ...
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Jul 1, 2019 · In the long list of identified candidate T-DNA insertions, researchers always put more attention on the ones that are inserted to gene regions ...
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A variety of strategies have been devised to generate and isolate mutants in known genes of Arabidopsis by T-DNA or transposon insertional mutagenesis. In these ...
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Feb 1, 2016 · A piece of T-DNA (more than 5-kbp fragment) is inserted randomly into genome, resulting in causing significant effects on the gene function.
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T-DNA trans- formation can be used as a tool for insertional mutagenesis and also serves as an efficient vehicle for delivering target genes into plant cells. T ...
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Nov 11, 2008 · Tandem insertions and their effect upon expression of flanking genomic sequences. We typically observed that two or more copies of T-DNA was ...
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Molecular analysis shows that the inserts are predominantly concatamers of T-DNAs arranged as direct and inverted repeats. More recently these 8000 lines have ...
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Infection of germinating seeds of Arabidopsis thaliana with Agrobacterium results in transformed lines in which the integrated T-DNA from Agrobacterium and ...
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