293 H Cells, In CD 293 - Thermo Fisher Scientific

293 H Cells, in CD 293Citations & References (1)Gibco™293 H Cells, in CD 293Gibco™ 293-H cells were cloned from the original 293 cell line and adapted to Gibco™ CD 293 Medium. The 293Read moreHave Questions?

Catalog Number	Quantity
11631017	1.5 mL

Catalog number 11631017Price (USD) - Quantity:1.5 mLGibco™ 293-H cells were cloned from the original 293 cell line and adapted to Gibco™ CD 293 Medium. The 293 cell line is a permanent line established from primary embryonic human kidney and transformed with sheared human adenovirus type 5 DNA. The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein. Gibco™ 293-H cells in Gibco™ CD 293 Medium feature:• Quick thaw into suspension culture directly in Gibco™ CD 293 Medium or Gibco™ 293 SFM II• Faster growth, good adherence, superior transfection efficiencies and high protein expression levels• Documented lineage from a low passage Master Cell Bank• Quality and performance testingQuick thaw into suspension culture directly in Gibco™ CD 293 Medium or Gibco™ 293 SFM IIEach vial containing 7.5 × 106 cells can be thawed directly into suspension culture in either Gibco™ CD 293 Medium or Gibco™ 293 SFM II supplemented with 4 mM L-glutamine or GlutaMAX™ reagent. Protocols are provided in the product manual.Faster growth, good adherence, superior transfection efficiencies and high protein expression levelsGibco™ 293-H cells were prepared from a clone selected for fast growth in serum-free medium, good adherence during plaque assays, superior transfection efficiencies and a high level of protein expression. Optimal transfection efficiencies are obtained with Lipofectamine™ 2000 or 293fectin™ transfection reagents.Documented lineage from a low passage Master Cell BankThe parental 293 cells were cloned by limiting dilution; the clone selected exhibited fast growth and high transfection efficiency. This clone was then re-cloned by limiting dilution; of these clones, the 293-H clone was then selected for its good adherence during plaque assays. The serum-free Master Cell Banks were prepared at approximately passage 25.Quality and performance testingEach lot of Gibco™ 293 H cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.Product UseFor Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.For Research Use Only. Not for use in diagnostic procedures.SpecificationsMedia RecommendationSFM (Serum-Free Media)Product TypeHEK 293 CellsQuantity1.5 mLShipping ConditionDry IceCell Line293HSpeciesHumanUnit SizeEachContents & Storage1 vial of 7.5 x 106 cellsStorage conditions: liquid nitrogen (vapor phase)Shipping conditions: frozen

Frequently asked questions (FAQs)

What is the difference between 293, 293H, 293F, and 293FT cells?

The 293 cell line is a permanent line established from primary embryonal human kidney transformed with sheared human adenovirus type 5 DNA (Graham et al., 1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein.

The FreeStyle 293-F cell line is a clone of the 293 cell line and is intended for use with the FreeStyle 293 Expression System. These cells are adapted to suspension culture in FreeStyle 293 Expression Medium.

The 293H cell line was also cloned from 293 cells, and like 293F cells can be grown as either suspension or adherent cultures. Neither 293F or 293H attach very well in SFM. In serum-supplemented medium, both attach better, but 293H attaches better than 293F. 293H is also better for transient transfection. 293F cells have a faster doubling time than 293H, and are better suited for selection of stables and large scale cultures.

The 293FT Cell Line is derived from the 293F Cell Line and stably expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid. Expression of the SV40 large T antigen is controlled by the human cytomegalo-virus (CMV) promoter and is high-level and constitutive.

Citations & References (1)

Citations & ReferencesAbstractEfficient intracellular assembly of papillomaviral vectors.Authors:Buck CB, Pastrana DV, Lowy DR, Schiller JT, Journal:J VirolPubMed ID:14694107Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More