Acetate Buffer (0.1 M, PH 5.0) Preparation And Recipe | AAT Bioquest

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Acetate Buffer (0.1 M, pH 5.0) Preparation and RecipeSodium acetate buffers are used for purification and precipitation of nucleic acids, as well as for protein crystallization and staining gels used in protein electrophoresis.To prepareL of Acetate Buffer (0.1 M, pH 5.0):Change the value in the textbox above to scale the recipe volumeTable 1. Required components
ComponentAmountConcentration
Sodium Acetate (mw: 82.03 g / mol)##
Acetic Acid (mw: 60.05 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium Acetate to the solution
  • Add # of Acetic Acid to the solution
  • Adjust solution to desired pH using 10N HCl (typically pH ≈ 5.0).
  • Add distilled water until the volume is 1 L.
AllPhysiological BufferpH BufferingSample PreparationBioAssaysMiscCell/Culture/Growth MediaGel ElectrophoresisReferencesThis online resource may be cited as follows
MLA"Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe." AAT Bioquest, Inc., 29 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.
APAAAT Bioquest, Inc. (2025, December 29). Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.
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This online resource has been cited in 1 publications, includingTea Waste as a Green Method in Removing Aluminum from Drinking WaterAuthors: Calkins, Sarah
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