ACQUITY UPLC HSS Columns : Waters - 예일테크

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ACQUITY UPLC® HSS C18 SB columns are designed specifically for chromatographers who routinely screen and categorize UPLC® column chemistries as part of their method development protocols. Intended for low pH separations which contain complex mixtures of basic and non-basic compunds, ACQUITY UPLC HSS C18 SB columns can produce separations that will be quite different in terms of selectivity as compared to those of most modern, high-coverage fully end-capped C18 stationary phases.

How was this accomplished? Waters materials scientists bonded a trifunctional C18 ligand at an intermediate ligand density [i.e., 1.6 µmol/m2] without endcapping. In general, the higher silanophilic activity will promote greater retention for basic compounds whereas the intermediate C18-ligand density will produce slightly shorter [or equivalent] retention for non-basic compounds as compared to most modern high coverage, fully end-capped C18 stationary phases. Chromatographically, this unique combination will provide a practical method development tool for complex UPLC separations.

Fast Separations of Tricyclic Antidepressants

Tricyclic antidepressants [TCAs] are secondary and tertiary amines and were commonly prescribed to patients suffering from depression or other conditions. TCAs are being replaced by selective serotonin reuptake inhibitors (SSRIs). ACQUITY UPLC HSS C18 SB columns retain basic compounds such as tricyclic antidepressants longer than fully endcapped stationary phases due to the enhanced silanol activity on the non-endcapped HSS particle.

ACQUITY UPLC HSS T3 Columns

ACQUITY UPLC® HSS T3 columns utilize Waters innovative and proprietary T3 bonding. This is the same advanced bonding process that is behind the industry-leading polar-compound retention, aqueous mobile-phase compatibility and ultra-low MS bleed of Atlantis® T3 HPLC columns. T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile-phase compatibility. The T3 endcapping is much more effective than traditional trimethyl silane [TMS] end-capping. This unique combination of bonding and endcapping provides superior polar compound retention and aqueous compatibility while also enhancing column performance, lifetime, peak shape and stability.

Although the rugged and efficient ACQUITY UPLC BEH particle provides a wide pH range and superior peak shapes, its hydrophilic nature does not promote polar compound retention. ACQUITY UPLC HSS T3 columns are designed to retain and separate polar organic compounds in reversed-phase UPLC® separations. When compared to ACQUITY UPLC BEH C18 columns, most compounds are more strongly retained on ACQUITY UPLC HSS T3 columns.

Superior Retention of Polar Compounds

ACQUITY UPLC HSS T3 columns retain polar compounds longer than ACQUITY UPLC BEH C18 columns and contain one of the most retentive sub-2 µm column chemistries commercially available.

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