Anti Bovine WC1 Antibody, Clone CC101 | Bio-Rad

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Mouse anti Bovine WC1

WC1 antibody | CC101 ISO 9001 Quality Assurance Manufacturing | Print

Mouse anti Bovine WC1

Product Type Monoclonal Antibody Clone CC101 Isotype IgG2a Specificity WC1

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Secondary Antibodies
References (19)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Unrivaled Technical Support 3 Formats Available: Alexa Fluor® 647 FITC Purified

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MCA1655GA Datasheet SDS SDS	C F IP	0.1 mg		Log in	- + Add to Cart Add to Quote
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Anti Bovine WC1 Antibody, clone CC101 thumbnail image 1

Anti Bovine WC1 Antibody, clone CC101 thumbnail image 2

Anti Bovine WC1 Antibody, clone CC101 gallery image 1 — Bovine peripheral blood lymphocytes stained with Mouse anti Bovine WC1 antibody, clone CC101 (**MCA1655G**) followed by Goat anti Mouse IgG (H/L):FITC (**STAR117F**).

Anti Bovine WC1 Antibody, clone CC101 gallery image 2 — **Published customer image:** Fitc conjugated Mouse anti Bovine WC1 antibody, clone CC101 (**MCA1655F**) used to assess WC1 ezpression on bovine cells by flow cytometry.Image caption:Representative dot plots. Gate strategy: (1) R1, lymphocyte gate used for WC1+ cells and (2) R2, gate used for NKp46+ cells. Dot plots from representative samples of WC1+IFN-γ+ in line A and NKp46+ IFN-γ+ in line B for non-infected and infected co-cultures (double positive events are represented in the upper right quadrant of each dot plot). Representative dot plots of NKp46+IFN-γ+ cells incubated with 5mg and 10mg lyophilized bacterial culture medium supernatant (CS) from Mb04-303 are depicted in line C.From: Blanco FC, Gravisaco MJ, Bigi MM, García EA, Marquez C, McNeil M, Jackson M, Bigi F.Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.Front Immunol. 2021 Jul 15;12:674643.doi: 10.3389/fimmu.2021.674643.This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Product Description

Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHugh et al. 1993).The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker et al. 1993). Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr et al. 1994).

Product Details

Target Species Bovine Species Cross-Reactivity

Target Species	Cross Reactivity
Pig
Sheep

N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Preparation Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant Buffer Solution Phosphate buffered saline Preservative Stabilisers 0.09% sodium azide (NaN3) Carrier Free Yes Immunogen Con A stimulated bovine lymphocytes Approx. Protein Concentrations IgG concentration 1.0 mg/ml Regulatory For research purposes only Guarantee 12 months from date of despatch

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Applications

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use10μl of the suggested working dilution to label 106 cells in 100μl

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)	STAR117D550	F IF WB	0.1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)	STAR117D650	F IF	0.1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	- +	Add to Cart Add to Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	- +	Add to Cart Add to Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:FITC

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Mouse IgG2a Negative Control	MCA929	F	100 Tests	Log in	- +	Add to Cart Add to Quote
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Description	Mouse IgG2a Negative Control

Product Specific References (19)

Source Reference

Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes. Immunology 81: 36-40.

References for WC1 antibody

Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle. Vet Immunol Immunopathol. 39: 25-48.
MacHugh, N. et al. (1993) Clustering of monoclonal antibodies recognizing different members of the WC1 gene family. Vet Immunol Immunopathol. 39: 155-60.
Crocker, G. et al. (1993) Analysis of the gamma/delta T cell restricted antigen WC1. Vet Immunol Immunopathol. 39: 137-44.
Lund, B. et al. (1993) Expression of T19 (WC1) molecules by ovine lymphocytes. Vet Immunol Immunopathol. 39: 145-53.
Schröder, A.C. & Hamann, J. (2005) The influence of technical factors on differential cell count in milk. J Dairy Res. 72: 153-8.
Patarroyo, J.H. et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus. Vet Parasitol. 166: 333-9.
Al-Mohammed Salem Kazem, T. et al. (2012) The Cellular Populations of Normal Camel (<i>Camelus dromedaries</i>) Milk Open Journal of Veterinary Medicine. 02 (04): 262-5.
Sedlak, C. et al. (2014) IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells. Dev Comp Immunol. 47: 115-22.

View The Latest Product References

Liu, X. et al. (2014) Crusted scabies is associated with increased IL-17 secretion by skin T cells. Parasite Immunol. 36: 594-604.
Al-Ashqar, R.A. et al. (2015) The CD markers of camel (Camelus dromedarius.) milk cells during mastitis: the LPAM-1 expression is an indication of possible mucosal nature of the cellular trafficking. Res Vet Sci. 99: 77-81.
Heiser, A. et al. (2015) Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving. J Dairy Sci. 98: 937-46.
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis. Sci Rep. 7 (1): 3441.
Hussen, J. et al. (2018) Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels. Pakistan Veterinary Journal. 38 (03): 231-236.
Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle. Vet Immunol Immunopathol. 232: 110167.
Blanco, F.C. et al. (2021) Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells. Front Immunol. 12: 674643.

Bioinformatics

UniProt P30205 Entrez Gene CD163L1 GO Terms GO:0016020 membrane GO:0005576 extracellular region GO:0005044 scavenger receptor activity

Batch/Lot Specific Datasheets

MCA1655GA

149995

1601

165739

171084

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Từ khóa » Bu Cc101

Anti Bovine WC1 Antibody, Clone CC101 | Bio-Rad

Mouse anti Bovine WC1

Quick Links:

Product Description

Product Details

Storage Information

Applications

Secondary Antibodies Available

Negative Isotype Controls Available

Product Specific References (19)

Source Reference

References for WC1 antibody

Further Reading

Bioinformatics

Batch/Lot Specific Datasheets

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