CAPS (0.5 M, PH 10.4) Preparation And Recipe | AAT Bioquest

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CAPS (0.5 M, pH 10.4) Preparation and RecipeCAPS is used commonly for Western Blot applications and other protein sorting procedures. The very high pH of this buffer allows for identification and sequencing of peptides and proteins with high isoelectric points (pl or IEP). To prepareL of CAPS (0.5 M, pH 10.4):Change the value in the textbox above to scale the recipe volumeTable 1. Required components
ComponentAmountConcentration
CAPS (mw: 221.32 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of CAPS to the solution
  • Adjust solution to desired pH using 10N NaOH.
  • Add dH2O until the volume is 1 L.
AllPhysiological BufferpH BufferingSample PreparationBioAssaysMiscCell/Culture/Growth MediaGel ElectrophoresisReferencesThis online resource may be cited as follows
MLA"Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe." AAT Bioquest, Inc., 30 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.
APAAAT Bioquest, Inc. (2025, December 30). Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.
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