CC-503 Cw92 Mt+ - Chlamydomonas Resource Center
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From George Witman, given to N.W. Gillham on a visit to Princeton, no accompanying letter, March 1978.
Phenotype: wall deficient
Original mutant isolated by D.R. Davies in the Ebersold/Levine 137c wild type background (equivalent to CC-125).
This is a very clean wall-deficient mutant, placed by Davies in his class C: cell walls are absent or produced in greatly reduced quantity compared to wild type. It forms flat, liquid colonies on agar, and the cells lyse in 1% solution of the nonionic detergent NP-40.
This strain was used for preparation of the DNA used for genomic sequencing.
Hyams J and Davies DR (1972) The induction and characterisation of cell wall mutants of Chlamydomonas reinhardi. Mut Res 14:381-389
Voigt J, Hinkelmann B, Harris EH (1997) Production of cell wall polypeptides by different cell wall mutants of the unicellular green alga Chlamydomonas reinhardtii. Microbiol Res 152:189-198
Hyams J and Davies DR (1972) The induction and characterisation of cell wall mutants of Chlamydomonas reinhardi. Mut Res 14:381-389
Voigt J, Hinkelmann B, Harris EH (1997) Production of cell wall polypeptides by different cell wall mutants of the unicellular green alga Chlamydomonas reinhardtii. Microbiol Res 152:189-198
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