Citrate Buffer (0.1 M, PH 6.0) Preparation And Recipe | AAT Bioquest

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Citrate Buffer (0.1 M, pH 6.0) Preparation and RecipeCitrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.Available for purchase here.To prepareL of Citrate Buffer (0.1 M, pH 6.0):Change the value in the textbox above to scale the recipe volumeTable 1. Required components
ComponentAmountConcentration
Sodium Citrate dihydrate (mw: 294.1 g / mol)##
Citric Acid (mw: 192.12 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium Citrate dihydrate to the solution
  • Add # of Citric Acid to the solution
  • Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).
  • Add distilled water until the volume is 1 L.
AllPhysiological BufferpH BufferingSample PreparationBioAssaysMiscCell/Culture/Growth MediaGel ElectrophoresisReferencesThis online resource may be cited as follows
MLA"Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe." AAT Bioquest, Inc., 30 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.
APAAAT Bioquest, Inc. (2025, December 30). Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.
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This online resource has been cited in 3 publications, includingInsoluble Retrogradation-Resistant Starch-Chitosan FoamsAuthors: Chen, Caini and Catchmark, Jeffrey MJournal: Journal of Polymers and the Environment (2025): 1--16Factors affecting stability of RNA--temperature, length, concentration, pH, and buffering speciesAuthors: Chheda, Urmi and Pradeepan, Selvi and Esposito, Edward and Strezsak, Steven and Fernandez-Delgado, Olivia and Kranz, JamesJournal: Journal of Pharmaceutical Sciences (2023)Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat modelsAuthors: Ratnaningtyas, NI and Ekowati, N and Husen, F and others,
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