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1405, Parc Technologique Blvd., 2nd Floor, Quebec, CanadaG1P 4P5

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(844) 629-5413

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Discover our work in life sciences with SPARQ, bringing patented HiLo-based optical sectioning to widefield microscopes for fast, confocal-like high-contrast imaging.

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Applications

Applications	Features	Benefits
Fixed cell imaging	- Multi-channel acquisition	SPARQ can be used with any fluorophore
Live cell imaging	- Low laser intensity (comparable to standard widefield illumination)	Reduces photobleaching and phototoxity, allowing for long imaging sessions
	- Fast 2D and 3D imaging	With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ
	- Multi-dimensional acquisition: Time lapse, z-stacks, multi-channels	Set your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images
	- Combinaison with transmitted light constrat methods	Combine your SPARQ images with any contrast modes, without even changing objectives
Tissue slice imaging	- High penetration depth	Since the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper
	- Modification of optical thickness	Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution
	- 3D imaging and reconstruction	Create high quality 3D images by rejecting out-of-focus fluorescence
Whole mount and cleared tissue imaging	- Large area stitching	Keep the same imaging speed regardless of the field of view. SPARQ works with any camera
	- Use different objective magnifications	Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter
	- Works with any kind of objectives	Use high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images
	- Keep high axial and lateral resolution	Improve the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy
In vivo imaging (on 2P)	- Can be used in combinaison with photo-stimulation	Use the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths)
	- Offers optical sectioning with visible lasers on a 2P optimized system	Increase the flexibilty of your 2P system by adding SPARQ and get visible light confocality
	- Can be used in vivo for positioning and other fluorescence uses	A one push button allows you to easily switch between SPARQ and other imaging modes

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