CprG Antibody (H36) - DSHB

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H36

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SKU: H36 Product Form : Supernatant 1.0 ml Frozen Cells 1 vial Growing Cells 1 vial

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Clone ID/Product Name: H36 Available to For-Profits: Yes Alternate Antibody Name: Gene Symbol: cprG Ab Isotype: MIgG1 Gene Name: Antibody Regi...

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Clone ID/Product Name: H36Available to For-Profits: YesAlternate Antibody Name: Gene Symbol: cprGAb Isotype: MIgG1Gene Name: Antibody Registry ID: AB_1553750 Uniprot ID: Q94504 RRID: Entrez Gene ID: 8621915 Clonality: MonoclonalImmunogen: Crude preparation of Dictyostelium membranes.Clone: Immunogen Sequence: Full length proteinMyeloma Strain: X63Epitope Mapped: YesAntigen Name: p46 cell surface protein excluded from macropinocytic cupEpitope Location or Sequence: N-acetylglucosamine-1-phosphateAlternate Antigen Name: Deposit Date: 2/5/2009Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 46 kDaDepositor: Cosson, P.Antigen Sequence: Depositor Institution: Centre Medical Universitaire, GenevaAntigen Species: DictyosteliumDepositor Notes: Host Species: mouseHybridoma Cells Available (Non-Profit): YesConfirmed Species Reactivity: DictyosteliumAdditional Information: H36 recognizes multiple proteins. The Cystein proteinase 7 (CP7) appears to be the main epitope of H36. H36 is also a marker of multilamellar bodies (MLB) where a different protein CDD (a protein containing a cytidine/deoxycytidylate deaminase (CDD) zinc-binding region) seems to be the target ( see [PMID: 27340834] for more details).Predicted Species Reactivity: Human Protein Atlas: Additional Characterization: Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot All cell products contain the antimicrobial ProClin. Click here for additional information. These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us. For your Materials & Methods section: H36 was deposited to the DSHB by Cosson, P. (DSHB Hybridoma Product H36) Storage and Handling Recommendations Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. Usage Recommendations The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

Initial Publication

IF References

WB References

IP References

FACS References

Epitope Map References

All References

Initial Publication

Selective membrane exclusion in phagocytic and macropinocytic cups.Cosson P Journal of cell science 119.Pt 19 (2006 Oct 1): 4079-87.

IF References

Selective membrane exclusion in phagocytic and macropinocytic cups.Cosson P Journal of cell science 119.Pt 19 (2006 Oct 1): 4079-87.

A role for adaptor protein-3 complex in the organization of the endocytic pathway in Dictyostelium.Cosson P Traffic (Copenhagen, Denmark) 7.11 (2006 Nov): 1528-38.

A LYST/beige homolog is involved in biogenesis of Dictyostelium secretory lysosomes.Cosson P Journal of cell science 120.Pt 14 (2007 Jul 15): 2338-43.

Immunofluorescence labeling of cell surface antigens in Dictyostelium.Cosson P BMC research notes 6. (2013 Aug 12): 317.

Lipid composition of multilamellar bodies secreted by Dictyostelium discoideum reveals their amoebal origin.Charette SJ Eukaryotic cell 12.10 (2013 Oct): 1326-34.

Effect of starvation on the endocytic pathway in Dictyostelium cells.Cosson P Eukaryotic cell 9.3 (2010 Mar): 387-92.

Amoeba-resisting bacteria found in multilamellar bodies secreted by Dictyostelium discoideum: social amoebae can also package bacteria.Charette SJ FEMS microbiology ecology 92.3 (2016 Mar): .

Identification of Proteins Associated with Multilamellar Bodies Produced by Dictyostelium discoideum.Charette SJ PloS one 11.6 (2016): e0158270.

Role of PKD2 in rheotaxis in Dictyostelium.Cosson P PloS one 9.2 (2014): e88682.