Dilution Calculator | Tocris Bioscience

The Tocris dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

Concentration 1 femtomolar picomolar nanomolar micromolar millimolar molar x Volume 1 nanoliter microliter milliliter liter = Concentration 2 femtomolar picomolar nanomolar micromolar millimolar molar x Volume 2 nanoliter microliter milliliter liter

The dilution calculator equation

The Tocris dilution calculator is based on the following equation:

Concentration(start) x Volume(start) = Concentration(final) x Volume(final)

This equation is commonly abbreviated as: C1V1 = C2V2

An example of a dilution calculation using the Tocris dilution calculator

What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?

Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:

1. Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
2. Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
3. Enter 20 into the Volume (final) box and select the correct unit (milliliter)
4. Press calculate
5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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