Effect Of CH4/O2 Ratio On Fatty Acid Profile And Polyhydroxybutyrate ...

Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

Understanding the role of heterotrophic-methanotrophic (H-Meth) communities is important for improvement of methane (CH4) oxidation capacities (MOC) particularly in conjunction with bio-product development in industrial bio-filters. Initially, a H-Meth consortium was established and enriched from marine sediments and characterized by next generation sequencing of the 16s rDNA gene. The enriched consortium was subjected to 10-50% CH4 (i.e., 0.20-1.6 CH4/O2 ratios) to study the effects on MOCs, biomass growth, fatty acid profiles and biopolymer (e.g. polyhydroxybutyrate; PHB) content. Methylocystis, Methylophaga and Pseudoxanthomonas dominated the H-Meth consortium. Culture enrichment of the H-Meth consortium resulted in 15-20-folds higher MOC compared to seed sediments. Increasing CH4 concentration (and decreased O2 levels) yielded higher MOCs, but did not improve total fatty acid contents. PHB contents varied between 2.5% and 8.5% independently of CH4/O2 ratios. The results suggest that H-Meth consortia could potentially be used in industrial bio-filters for production of biopolymer/biofuel precursors from CH4.

Keywords: Bioproducts; Fatty acids; Heterotrophs; Methane remediation; Microbial community; Polyhydroxybutyrate.

Copyright © 2015 Elsevier Ltd. All rights reserved.

PubMed Disclaimer

Publication types

• Research Support, Non-U.S. Gov't Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

MeSH terms

• Biomass Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Bioreactors / microbiology Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• DNA, Ribosomal / genetics Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Fatty Acids / analysis Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Fatty Acids / chemistry* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Geologic Sediments / microbiology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• High-Throughput Nucleotide Sequencing Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Hydroxybutyrates / analysis Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Hydroxybutyrates / chemistry* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Methane / analysis* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Methylocystaceae / chemistry Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Methylocystaceae / growth & development* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Methylocystaceae / isolation & purification Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Microbial Consortia* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Oxidation-Reduction Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Oxygen / analysis* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Polyesters / analysis Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Polyesters / chemistry* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

Substances

• DNA, Ribosomal Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Fatty Acids Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Hydroxybutyrates Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Polyesters Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• poly-beta-hydroxybutyrate Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Methane Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Oxygen Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

LinkOut - more resources

• Full Text Sources

  • Elsevier Science