ExpiCHO-S™ Cells - Thermo Fisher
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Catalog Number | Format |
---|---|
A29127 | 1 vial |
A29132 | 6-vial pack |
Features of ExpiCHO-S Cells include:
- Suspension culture cells adapted in ExpiCHO Expression Medium
- Demonstrated yields of up to 3 g of protein per L of transfected culture as part of the ExpiCHO expression system
- Rapid doubling times of approximately 17 hours
- Consistent transient expression performance for over 20 passages after thaw
- High cell viability and productivity for greater than 10 days post-transfection as part of the ExpiCHO expression system
- Enable rapid, high-yield transient protein production, allowing labs developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells per mL in ExpiCHO Expression Medium and 10% DMSO. The cells should be thawed directly into ExpiCHO Expression Medium. The 6-vial multipack eliminates the need to expand and bank the cells in the lab.
Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL up to 5 L when using a Nalgene 5-L angled-bottom flask. Protein harvest can be performed at 5 to 14 days post-transfection, depending on the protein expressed.
Reference
1. Wurm, FM. CHO Quasispecies—Implications for Manufacturing Processes (2013) Processes 1:296-311.
For Research Use Only. Not for use in diagnostic procedures.SpecificationsCell LineCHO-S, SFM AdaptedFormCryopreservedSpeciesHamsterFormat1 vialFor Use With (Application)ExpressionNo. of Cells1 x 107Product LineExpiCHO™Product TypeCHO-S CellQuantity1 mLShipping ConditionDry IceUnit SizeEachContents & Storage1 x 107 cells/mLStore in liquid nitrogen.Từ khóa » Cho Bsl
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