Glycine-HCl Buffer (0.1 M, PH 3.0) Preparation And Recipe

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Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and RecipeThis low-pH buffer is used extensively with affinity chromatography, particularly with proteins and antibodies. It is also used for cell culture and other biological applications. To prepareL of Glycine-HCl Buffer (0.1 M, pH 3.0):Change the value in the textbox above to scale the recipe volumeTable 1. Required components
ComponentAmountConcentration
Glycine (mw: 75.07 g / mol)##
Hydrochloric acid (mw: 36.46 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Glycine to the solution
  • Add # of Hydrochloric acid to the solution
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until the volume is 1 L.
AllPhysiological BufferpH BufferingSample PreparationBioAssaysMiscCell/Culture/Growth MediaGel ElectrophoresisReferencesThis online resource may be cited as follows
MLA"Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe." AAT Bioquest, Inc., 12 Mar. 2026, https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.
APAAAT Bioquest, Inc. (2026, March 12). Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.
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