Guanidine HCl Datasheet - Selleck Chemicals

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Guanidine HCl

Catalog No.S4070 Batch:S407001

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Technical Data

Formula

CH5N3.HCl
Molecular Weight 95.53 CAS No. 50-01-1
Solubility (25°C)* In vitro DMSO 19 mg/mL (198.89 mM)
Water 19 mg/mL (198.89 mM)
Ethanol 6 mg/mL (62.8 mM)
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension CMC-NA ≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
In vitro Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by this compound provides several important parameters regarding the mechanism of conformational stability of proteins. [1] It at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. This chemical (> 1 M) causes co-operative unfolding of the molten globule state. [2] It is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GH2Oapp. values of this compound are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. [3] It at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM of this chemical in growth media cures [PSI+] and other prions of yeast. [4] 5 mM of this compound significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. It also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. [5]

Protocol (from reference)

References

  • https://pubmed.ncbi.nlm.nih.gov/3773761/
  • https://pubmed.ncbi.nlm.nih.gov/8389881/
  • https://pubmed.ncbi.nlm.nih.gov/4416801/
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC26647/
  • https://pubmed.ncbi.nlm.nih.gov/11375656/

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Selleck's Guanidine HCl Has Been Cited by 1 Publication

Cevipabulin-tubulin complex reveals a novel agent binding site on α-tubulin with tubulin degradation effect [ Sci Adv, 2021, 7(21)eabg4168] PubMed: 34138737

RETURN POLICY Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.

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