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Search This free script provided by JavaScript Kit | H441 (NCI-H441) cells Catalog #: C0016019 Shipping advice: Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details | Frozen vial | USD420.0  | Disease: Papillary adenocarcinoma Origin: The H441 cell line was derived in 1982 from the pericardial fluid of a patient with papillary adenocarcinoma of the lung. Species: Homo sapiens, human Tissue: Lung Morphology: Epithelial Properties: Adherent Cytogenic data: Modal number = 52; range = 44 to 59. Patient: Male. Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37°C. A subcultivation ratio of 1:3 to 1:8 is recommended. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 9,13 D5S818:11,12 D7S820:10 THO1:9.3 TPOX: 8,10 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: H441 (AddexBio C0016019) References for H441 cells: 1. Bepler G, Bading H, Heimann B, Kiefer P, Havemann K, Moelling K. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene. 1989;4(1):45-50. PMID: 2536917 Certificate of Analysis/MSDS is available upon request with lot number |
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