HT29-Lucia™ AhR Reporter Cells - InvivoGen

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AhR Reporter HT29 Cells

AhR-Lucia reporter colon adenocarcinoma

HT29-Lucia™ AhR Cells

• Cat.code: ht2l-ahr
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$1,436 Cell format AhR CellsAdditional vial * Unit Size 3-7 x 10^6 cells Quantity *Save on additional vials when you buy the matching cell reference. Bulk / Custom order Need a quote?

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This product is for internal research use for non-profit recipients only

ABOUT

Human HT29 colon adenocarcinoma - AhR reporter cells

HT29-Lucia™ AhR cells are engineered from the human HT-29 colon adenocarcinoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein.

AhR is a ligand-dependent transcriptional factor widely expressed in barrier tissues. Notably, AhR plays an important role in gut microbiota and the host's immune homeostasis [1].

HT-29 cells are characterized by:

• Endogenous AhR expression [2]
• Ability to differentiate into intestinal enterocyte-like cells and mucus-producing cells [3]

Therefore, HT29-Lucia™ AhR cells are highly relevant for studying intestinal microbiota-related ligands for AhR.

HT29-Lucia™ AhR cells express the secreted Lucia luciferase reporter gene under the control of a minimal promoter coupled with the human Cyp1a1 gene's entire regulatory sequence, which contains six dioxin-responsive elements (DREs).As a result, these cells allow studying the AhR genomic signaling pathway, by monitoring the activity of Lucia luciferase in the cell culture supernatant when using the QUANTI-Luc™ 4 Lucia/Gaussia detection reagent.

HT29-Lucia™ AhR cells are resistan to Zeocin®.

Read our review on AhR's key role in intestinal microbiota and immunity.

References

1. Lamas B et al.< 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.2. Park JH. et al., 2018. Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep. 39(4):1930-8. 3 artínez-Maqueda D. et al. 2015. HT29 Cell Line. Bioactives on Health: in vitro and ex vivo models. Springer. Chapt 1.

Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

Image AhR signaling pathway

SPECIFICATIONS

Specifications

Tested applications

Lucia luciferase activity assay

Growth properties Adherent Tissue origin Human colorectal carcinoma Reporter gene Lucia® Antibiotic resistance Zeocin® Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

• Product: HT29-Lucia™ AhR Cells
• Cat code: ht2l-ahr
• Quantity: 3-7 x 10^6 cells

Includes:

1 ml Zeocin® (100 mg/ml)1 ml Normocin™ (50 mg/ml)1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)

Shipping & Storage

• Shipping method: Dry ice

Storage:

• Liquid nitrogen vapor
Stability: 20 passages

Details

The aryl hydrocarborn receptor (AhR) is a ligand-dependent transcriptional factor widely expressed in barrier tissues [1]. AhR plays a key role in gut-microbiota and host’s immune homeostasis, not only in the intestine but also at distant sites [1].Besides xenobiotics, including the prototypic AhR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a variety of dietary-derived AhR ligands have been identified, many of which are byproducts of tryptophan (Trp) metabolism [2].

Inactive AhR resides in the cytoplasm within a Hsp90:XAP2:p23:Src protein complex. The AhR canonical genomic signaling pathway occurs as follows: upon ligand binding, the complex undergoes conformational changes and translocates into the nucleus. AhR heterodimerizes with AhR nuclear translocator (ARNT) before binding to dioxin response elements (DREs) in the upstream regulatory regions of AhR target genes, such as the cytochrome P450-dependent monooxygenase Cyp1a1, the AhR repressor (AhRR), and the IL-22 interleukin.

Of note, non-canonical AhR signaling pathways have also been reported, either at the genomic level through association with other transcription factors (e.g. NF-κB), or at the non-genomic level (e.g. through the release of the Src kinase) [2,3].

References

1. Lamas B. et al. 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.2. Gao J. et al. 2018. Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism. Front. Cell. Infect. Microbiol. 8:13.3. Park JH. et al., 2018. Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep. 39(4):1930-8.

DOCUMENTS

Documents

HT29-Lucia™ AhR Cells

Technical Data Sheet

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Validation Data Sheet

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Safety Data Sheet

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Certificate of analysis

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Citations

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