Hu Et Al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(12): 4613 - FlyBase

• Tools
  • Tools Overview & Help
  • Query by symbols/IDs
    • Batch Download
    • Sequence Downloader
    • ID Validator
    • Feature Mapper
  • Search/Browse Portals
    • QuickSearch
    • Vocabularies
    • QueryBuilder
    • CytoSearch
    • Sequenced Species
    • Interactions Browser
    • ImageBrowse
  • Genomics Tools
    • BLAST
    • Fly BLAST @ Alliance
    • JBrowse
    • CytoSearch
    • Feature Mapper
    • Chromosome Maps
    • Synteny Table
    • Coordinates Converter
    • Sequence Downloader
    • modENCODE RNA-Seq
      • Overview
      • RNA-Seq Similarity
      • RNA-Seq Profile
      • RNA-Seq By Region
      • JBrowse
  • Submit Data
    • Fast-Track Your Paper
    • Submit Personal Communication
    • Feedback on Gene Snapshots
• Downloads
  • Overview
  • Current Release
  • Archived Data
  • Map Conversion
  • Releases (FTP)
• Links
  • External Resources
  • Model Organisms (MODs)
    • Alliance of Genome Resources
    • BeeBase
    • DictyBase
    • EcoCyc
    • Gramene
    • MGI
    • PomBase
    • Pseudobase
    • RGD
    • SGD
    • TAIR
    • VectorBase
    • WormBase
    • Xenbase
    • ZFIN
  • Stock Collections
    • Bloomington Drosophila Stock Center (BDSC)
    • FlyORF
    • Korea Drosophila Resource Center (KDRC)
    • Kyoto Stock Center
    • NIG-FLY
    • Tsinghua Fly Center (THFC)
    • Vienna Drosophila Resource Center (VDRC)
  • BDGP
  • DGRC
  • DRSC/TRiP
  • DIS by issue
  • FlyBook
  • FlyExpress
  • Interactive Fly
  • Virtual Fly Brain
  • FlyCyc
• Community
  • Fast-Track Your Paper
  • FlyBase Community Advisory Group
  • Fly Lab List
  • Recent Papers With Technical Advances
  • Gene Snapshots
  • FlyBase Forum
  • Newsletter
  • FlySlack Community
  • Mastodon
  • Bluesky
  • X (formerly Twitter)
  • Fly Board
  • NIH Funding
• About
  • Release Notes
  • New In This Release
  • Release Schedule
  • FlyBase Positions
  • Citing FlyBase
  • FlyBase Licensing
  • FlyBase Consortium
  • FlyBase Publications
  • FlyBase Presentations
  • Grants Supporting FlyBase
• Help
  • Contact FlyBase
  • FlyBase Wiki:
  • Help Index
  • Tool help
  • Report help
  • FlyBase FAQ
  • Info for Authors
    • Author guidelines
    • Citing FlyBase
    • Fast-Track Your Paper Help
    • Gene Snapshots
    • Personal communications
  • Linking to/from FlyBase
  • Nomenclature
  • Curation documentation
    • Controlled Vocabularies in FlyBase
    • Gene Model Annotation
    • Gene Ontology (GO) Annotation
    • Nontraditional alleles
    • Vectors & Constructs
  • New to Flies?
  • Video Tutorials
  • Programmatic Access to FlyBase: APIs, etc.

FlyBase in AWS Open Data FlyBase NIH Grant Terminated Previous Next FB2025_05 , released December 11, 2025 Open Close Reference Citation Hu, H., Li, M., Labrador, J.P., McEwen, J., Lai, E.C., Goodman, C.S., Bashaw, G.J. (2005). Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. Proc. Natl. Acad. Sci. U.S.A. 102(12): 4613--4618. FlyBase ID FBrf0191476 Publication Type Research paper Abstract The regulators of the Rho-family GTPases, GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs), play important roles in axon guidance. By means of a functional genomic study of the Rho-family GEFs and GAPs in Drosophila, we have identified a Rho-family GAP, CrossGAP (CrGAP), which is involved in Roundabout (Robo) receptor-mediated repulsive axon guidance. CrGAP physically associates with the Robo receptor. Too much or too little CrGAP activity leads to defects in Robo-mediated repulsion at the midline choice point. The CrGAP gain-of-function phenotype mimics the loss-of-function phenotypes of both Robo and Rac. Dosage-sensitive genetic interactions among CrGAP, Robo, and Rac support a model in which CrGAP transduces signals downstream of Robo receptor to regulate Rac-dependent cytoskeletal changes. PubMed ID 15755809 PubMed Central ID PMC555501 (PMC) (EuropePMC) DOI 10.1073/pnas.0409325102 Related Publication(s) Supplementary material

[title not yet available] [FBrf0191477]

Associated Information Comments Associated Files Other Information Secondary IDs Language of Publication English Additional Languages of Abstract Parent Publication Publication Type Journal Abbreviation Proc. Natl. Acad. Sci. U.S.A. Title Proceedings of the National Academy of Sciences of the United States of America Publication Year 1915- ISBN/ISSN 0027-8424 Data From Reference Alleles (19) Export to HitList

• Cdc42N17.UAS
• CdGAPrRNAi.UAS
• MtlΔ
• Rac1J10
• Rac1N17.UAS
• Rac1UAS.cLa
• RacGAP84CUAS.cRa
• Rho1RNAi.UAS.cBa
• Rho1UAS.cMa
• RhoGAP19DRNAi.UAS.cBa
• RhoGAP93BEY06358
• RhoGAP93BRNAi.UAS.cBa
• RhoGAP93BUAS.cHa
• RhoGAP100FRNAi.UAS.cBa
• robo15
• Scer\GAL4elav.PLu
• Scer\GAL4GMR.PF
• sli1
• tumRNAi.UAS.cBa

Genes (14) Export to HitList

• Cdc42
• CdGAPr
• Mtl
• Rac1
• RacGAP84C
• Rho1
• RhoGAP19D
• RhoGAP93B
• RhoGAP100F
• RhoGAPp190
• robo1
• Scer\GAL4
• sli
• tum

Physical Interactions (3) Export to HitList

• RhoGAP93B - robo1

Cell Lines (1) Export to HitList

• S2-unspecified

Natural transposons (1) Export to HitList

• P-element

Insertions (4) Export to HitList

• P{GAL4-elav.L}3A
• P{UAS-CrGAP.H}28
• P{UAS-CrGAP.H}33
• P{UAS-CrGAP.H}37

Experimental Tools (1) Export to HitList

• UASt

Transgenic Constructs (13) Export to HitList

• P{GAL4-elav.L}
• P{GAL4-ninaE.GMR}
• P{UAS-Cdc42.N17}
• P{UAS-CdGAPr-RNAi}
• P{UAS-CrGAP.H}
• P{UAS-Rac1.L}
• P{UAS-Rac1.N17}
• P{UAS-Rho1.RNAi.B}
• P{UAS-Rho1.Sph}
• P{UAS-RhoGAP19D.RNAi.B}
• P{UAS-RhoGAP93B.RNAi.B}
• P{UAS-RhoGAP100F.RNAi.B}
• P{UAS-tum.RNAi.B}

Report Sections

Open Close

• Reference
• Associated Information
• Other Information
• Parent Publication
• Data From Reference