Hui-Ling Chen | National Taiwan University | 69 Publications - Typeset

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Hui-Ling Chen

National Taiwan University

70 Papers651 Citations

Hui-Ling Chen is an academic researcher from National Taiwan University. The author has contributed to research in topics: Medicine & Hepatocyte. The author has an hindex of 25, co-authored 69 publications. Previous affiliations of Hui-Ling Chen include Kobe University.

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Papers

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Activation of phosphatidylinositol 3-kinase/Akt signaling pathway mediates acquired resistance to sorafenib in hepatocellular carcinoma cells. J Pharmacol Exp Ther 337: 155-161

Kuen-Feng Chen,Hui-Ling Chen,Wei-Tien Tai,Wen-Chi Feng,Chih-Hung Hsu,Pei-Jer Chen,Ann-Lii Cheng +6 more - 01 Jan 2011Show LessAbstract: Hepatocellular carcinoma (HCC) is one of the most common potentially lethal human malignancies worldwide. Sorafenib, a tyrosine kinase inhibitor, was recently approved by the United States Food and Drug Administration for HCC. In this study, we established two sorafenib-resistant HCC cell lines from Huh7, a human HCC cell line, by long-term exposure of cells to sorafenib. Sorafenib induced significant apoptosis in Huh7 cells; however, Huh7-R1 and Huh7-R2 showed significant resistance to sorafenib-induced apoptosis at the clinical relevant concentrations (up to 10 μM). Thorough comparisons of the molecular changes between Huh7 and resistant cells showed that the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway played a significant role in mediating acquired resistance to sorafenib in Huh7-R1 and Huh7-R2 cells. Phospho-Akt and p85 (a regulatory subunit of PI3K) were up-regulated, whereas tumor suppressor phosphatase and tensin homolog were down-regulated in these resistant cells. In addition, ectopic expression of constitutive Akt in Huh7 demonstrated similar resistance to sorafenib. The knockdown of Akt by RNA interference reversed resistance to sorafenib in Huh7-R1 cells, indicating the importance of Akt in drug sensitivity. Furthermore, the combination of 8-[4-(1-aminocyclobutyl)phenyl]-9-phenyl-1,2,4-triazolo[3,4-f][1,6]naphthyridin-3(2H)-one dihydrochloride (MK-2206), a novel allosteric Akt inhibitor, and sorafenib restored the sensitivity of resistant cells to sorafenib-induced apoptosis. In conclusion, activation of PI3K/Akt signaling pathway mediates acquired resistance to sorafenib in HCC, and the combination of sorafenib and MK-2206, an Akt inhibitor, overcomes the resistance at clinical achievable concentrations....read moreread less234Podcast•Journal Article•DOI

Porcine Spleen Deoxyribonuclease II COVALENT STRUCTURE, cDNA SEQUENCE, MOLECULAR CLONING, AND GENE EXPRESSION

Cheng-Ching Wang,Shao-Chun Lu,Hui-Ling Chen,Ta-Hsiu Liao +3 moreNational Taiwan University - 03 Jul 1998 - Journal of Biological Chemistry Show LessTL;DR: All three chains in the sequence α1, β, and α2 are coded by the same cDNA, and when Chinese hamster ovary cells were transfected with a cloned plasmid with an inserted cDNA fragment encoding the entire reading frame, the expressed protein was released into the growth medium as an active form of DNase II....read moreread less44Journal Article•DOI

Long-term culture of hepatocytes from human adults.

Hui-Ling Chen,Hui-Lin Wu,Chun-Chun Fon,Pei-Jer Chen,Ming-Yang Lai,Ding-Shinn Chen +5 moreNational Taiwan University - 01 Nov 1998 - Journal of Biomedical Science Show LessTL;DR: A long-term culture of hepatocytes from human adults is established, which will be useful for basic studies of liver physiology such as metabolism and morphogenesis, as well as for other applications in the study of infectious hepatitis, pharmacology, pharmacokinetics, and toxicology....read moreread less42Podcast•Journal Article•DOI

Ribavirin Up-Regulates the Activity of Double-Stranded RNA-Activated Protein Kinase and Enhances the Action of Interferon-a against Hepatitis C Virus

Wei Liang Liu,Wen Cheng Su,Ching Wei Cheng,Lih Hwa Hwang,Chih Chiang Wang,Hui-Ling Chen,Ding-Shinn Chen,Ming Yang Lai +7 moreNational Taiwan University - 01 Aug 2007 - The Journal of Infectious Diseases Show LessTL;DR: Results suggest that the anti-HCV action of ribavirin is partly attributable to its ability to up-regulate PKR activity, supporting the importance of PKR in the control of HCV-RNA replication....read moreread less37PDFPodcast•Journal Article•DOI

Secondary activation of c-abl may be related to translocation to the nucleolar organizer region in an in vitro cultured rat leukemia cell line (K3D)

Rei Takahashi,Koichiro Mihara,Sakan Maeda,Toshimasa Yamaguchi,Hui-Ling Chen,Nobuo Aoyama,Shinichi Murao,Masakazu Hatanaka,Taketoshi Sugiyama +8 more - 01 Feb 1986 - Proceedings of the National Academy of S... Show LessTL;DR: Evidence is provided of secondary c-onc activation during karyotypic evolution of cloned malignant cells in K3D, which was one of the cloned cell lines of 7,12-dimethylbenz[a]anthracene-induced rat erythroblastic leukemia....read moreread less25Podcast1234567891011121314...