Human Epidermal Keratinocytes, Neonatal (HEKn)

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Human Epidermal Keratinocytes, neonatal (HEKn)ShareProduct ImageCitations & References (1)Gibco™Human Epidermal Keratinocytes, neonatal (HEKn)Primary Normal Human Epidermal Keratinocytes, neonatal (HEKn) are isolated from neonatal foreskin and cryopreserved at the end of the primaryRead moreHave Questions?
Catalog NumberQuantity
C0015C1 vial
Catalog number C0015CPrice (USD) - Quantity:1 vialPrimary Normal Human Epidermal Keratinocytes, neonatal (HEKn) are isolated from neonatal foreskin and cryopreserved at the end of the primary culture.The cells are:• Performance tested—at least 30 population doublings when cultured in EpiLife Medium supplemented with HKGS (for HEKn) or Supplement S7 (for HEKn-AOF)• Guaranteed to be ≥ 70% viable• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 virusesEach vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing 10% DMSO.For Research Use Only. Not for use in diagnostic procedures.SpecificationsDonor SourceSingle DonorNo. of Cells≥500000Product LineCascade BiologicsProduct TypeHuman Epidermal KeratinocyteQuantity1 vialTested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1AgeNewborn (14 days or less)Cell TypeKeratinocyte CellsCulture TypeAdherent Cell CultureFormCryopreservedSpeciesHumanUnit SizeEachContents & StorageStore in liquid nitrogen.

Frequently asked questions (FAQs)

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs

Citations & References (1)

Citations & ReferencesAbstractHigh Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter.Authors:Apt D, Watts RM, Suske G, Bernard HU, Journal:VirologyPubMed ID:8862423Gene expression of human papillomavirus type 16 (HPV-16) and other HPV types is epithelial specific. Specificity is brought about by synergism between several different transcription factors that seem to occur ubiquitously but differ qualitatively and quantitatively between cells in which HPV genomes are transcriptionally active or inactive. Here, we report ... More

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