Lambda DNA - Thermo Fisher Scientific

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Lambda DNAShareLambda DNAThermo Scientific™Lambda DNAThermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12Read moreHave Questions?
Catalog NumberQuantity
SD0011500 μg
Catalog number SD0011Price (USD) - Quantity:500 μgRequest bulk or custom formatThermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.FeaturesPhage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is used. The DNA sequence used to construct a phage alpha restriction map includes these mutations.Applications• Activity and specificity assays of restriction enzymes• Preparation of DNA molecular weight standards• CloningGenBank/EMBL Accession NumbersNC_001416, M17233, M24325, V00636, X00906For Research Use Only. Not for use in diagnostic procedures.SpecificationsConcentration0.3 μg/μLFinal Product TypeDNAFor Use With (Application)Cloning/Nucleic Acid Gel ElectrophoresisIncludes[other]Product TypeLambda DNAQuantity500 μgSample TypeDNASpeciesLambda phageUnit Size500 µg

Frequently asked questions (FAQs)

Which E. coli strain is lambda DNA (Cat. No. SD0011) isolated from?

Lambda DNA (Cat. No. SD0011) is isolated from a heat-inducible lysogenic E. coli W3110 strain.

What concentration is lambda DNA (Cat. No. SD0011) provided at?

Lambda DNA (Cat. No. SD0011) is provided at a concentration of 0.3 µg/µL.

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