Mechanotransducing Ion Channels In C6 Glioma Cells - PubMed

Clipboard, Search History, and several other advanced features are temporarily unavailable. Skip to main page content

The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation Search: Search Advanced Clipboard Save Email Send to

• Clipboard
• My Bibliography
• Collections
• Citation manager

Display options Display options Format Abstract PubMed PMID

Save citation to file

Format: Summary (text) PubMed PMID Abstract (text) CSV Create file Cancel

Email citation

Email address has not been verified. Go to My NCBI account settings to confirm your email and then refresh this page. To: Subject: Body: Format: Summary Summary (text) Abstract Abstract (text) MeSH and other data Send email Cancel

Add to Collections

• Create a new collection
• Add to an existing collection

Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an error Please try again Add Cancel

Add to My Bibliography

• My Bibliography

Unable to load your delegates due to an error Please try again Add Cancel

Your saved search

Name of saved search: Search terms: Test search terms Would you like email updates of new search results? Saved Search Alert Radio Buttons

• Yes
• No

Email: (change) Frequency: Monthly Weekly Daily Which day? The first Sunday The first Monday The first Tuesday The first Wednesday The first Thursday The first Friday The first Saturday The first day The first weekday Which day? Sunday Monday Tuesday Wednesday Thursday Friday Saturday Report format: Summary Summary (text) Abstract Abstract (text) PubMed Send at most: 1 item 5 items 10 items 20 items 50 items 100 items 200 items Send even when there aren't any new results Optional text in email: Save Cancel

Create a file for external citation management software

Create file Cancel

Your RSS Feed

Name of RSS Feed: Number of items displayed: 5 10 15 20 50 100 Create RSS Cancel RSS Link Copy

Full text links

Wiley Full text links

Actions

CiteCollectionsAdd to Collections

• Create a new collection
• Add to an existing collection

Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an errorPlease try again Add Cancel PermalinkPermalinkCopyDisplay options Display options Format AbstractPubMedPMID

Page navigation

• Title & authors
• Abstract
• Publication types
• MeSH terms
• Substances
• LinkOut - more resources

Title & authors Abstract Publication types MeSH terms Substances LinkOut - more resources Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

Mechanotransducing (MS) ion channels and images of the patch membrane were studied in cell-attached patches in C6 glioma cells. MS channel density was approximately 0.08 to 0.5 channels/microns2, channel conductance was approximately 40 pS (at -40 mV), and the reversal potential was +15 mV. Replacement of NaCl with KCl, CsCl, or Na gluconate in the pipette solution was without substantial effect on the current-voltage relationship. Replacement of NaCl with NMDG (N-Methyl-D-Glucamine) Cl or reducing NaCl decreased the amplitude of inward currents at negative membrane potentials and caused the reversal potential to shift in the negative direction. Rapid application of suction to the back of the pipette usually elicited a fast (< 0.1 s) appearance of channel activity. The peak (phasic) in channel activity was followed by a decrease to a constant (tonic) level of activity. The reduction in channel activity--called adaptation--was reduced at depolarizing membrane potentials and disappeared if too much pressure was applied. Positive pressure caused the patch membrane to curve toward the pipette tip, move in the direction of the tip, and evoke MS channel activity. Removal of the positive pressure caused the patch to move back to the original position. Conversely, negative pressure caused the patch membrane to curve away from the pipette tip, move away from the tip, and elicit MS channel activity. Gigohm seal resistances were always maintained during translational movement of the patch membrane. Tonic MS channel activity was not associated with translational movements of the patch membrane. Phasic and tonic channel activity were independent of the sign of curvature of the patch membrane. C6 glioma cells have rapidly adapting voltage-dependent MS ion channels, which are non-selective for monovalent cations, and belong to the stretch-activating class of mechanosensory ion channels. Adaptation in MS channels may allow the cell to limit the influx of cations in response to mechanical input. The selective loss of adaptation suggests that the MS channel's gate receives input from two sources. A minimal viscoelastic mechanical model of adaptation and two alternative models for translational movement of the patch are presented.

PubMed Disclaimer

Publication types

• Research Support, U.S. Gov't, Non-P.H.S. Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

MeSH terms

• Adaptation, Physiological Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Animals Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Brain Neoplasms / physiopathology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Electrophysiology Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Glioma / physiopathology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Ion Channel Gating / physiology Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Ion Channels / physiology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Membrane Potentials / physiology Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Models, Biological Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Patch-Clamp Techniques Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Perfusion Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Pressure Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Rats Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Signal Transduction / physiology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Tumor Cells, Cultured Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

Substances

• Ion Channels Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

LinkOut - more resources

• Full Text Sources

  • Wiley

• Medical

  • MedlinePlus Health Information

Full text links [x] Wiley [x] Cite Copy Download .nbib .nbib Format: AMA APA MLA NLM Send To

• Clipboard
• Email
• Save
• My Bibliography
• Collections
• Citation Manager

[x]

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.