N-PER™ Neuronal Protein Extraction Reagent

N-PER™ Neuronal Protein Extraction ReagentCitations & References (3)Thermo Scientific™N-PER™ Neuronal Protein Extraction ReagentThermo Scientific N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins fromRead moreHave Questions?

Catalog Number	Quantity
87792	100 mL

Catalog number 87792Price (USD) - Quantity:100 mLRequest bulk or custom formatThermo Scientific N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons.Features of N-PER Neuronal Protein Extraction Reagent:• Optimized—efficient total protein extraction, including membrane proteins, from brain tissue or primary cultured neurons• Gentle—preserves protein function without compromising yield• Versatile—can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors• Compatible—extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methodsNeuron cell lysates, such as brain tissue extracts, that are prepared using the N-PER Reagent are suitable for enzymatic activity assays (e.g., phosphatase, kinase, ATPase assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90 μg of protein per mg of brain tissue or 300 μg of total protein from 106 primary neuronal cells.Applications:• Isolate functional proteins from neuronal tissues or primary neuronal cells• Study enzymatic activity assays specific to neuronal tissue• Generate lysates from neuronal tissues for biomarker analysisTypical neuron structures include the cell body (soma), dendrites and an axon. The unique morphology and rich sphingolipid and cholesterol composition of neuron cell membranes creates challenges in preparing neuronal protein extracts. N-PER Neuronal Protein Extraction Reagent is specifically formulated to overcome the challenges of extracting functional native proteins from brain or other neuronal tissue and primary cultured neurons while increasing protein yield compared to other extraction methods.The mild formulation of the N-PER Cell Lysis Reagent is ideal for downstream applications requiring active neuronal proteins. In addition, the N-PER Reagent is compatible with many applications including enzyme assays (e.g., phosphatase, kinase), immunoassays, various chromatography procedures and electrophoresis. Thermo Scientific Halt Protease Inhibitor Cocktail or Phosphatase Inhibitor Cocktail can be added just before use to prevent proteolysis or to offer additional protection from the high phosphatase activity normally present in brain tissue.For each extraction, a ratio of 1g of tissue to 10 mL of N-PER Reagent or 0.5-1 mL N-PER Reagent per 10 cm petri dish of primary cultured cells is recommended.More Product Data• Optimized protein extraction reagent for neuronal tissue and primary cellsRelated ProductsPierce™ Primary Neuron Isolation KitFor Research Use Only. Not for use in diagnostic procedures.SpecificationsFormatLiquidQuantity100 mLVolume (Metric)100 mLProduct LineNE-PERProduct TypeNeuronal Protein Extraction ReagentUnit SizeEachContents & StorageUpon receipt store product at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for the N-PER Neuronal Protein Extraction Reagent?

The N-PER Neuronal Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What lysis buffers do you offer for mammalian cells?

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells. Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Citations & References (3)

Citations & ReferencesAbstractCo-transplantation of GDNF-overexpressing neural stem cells and fetal dopaminergic neurons mitigates motor symptoms in a rat model of Parkinson's disease.Authors:Deng X, Liang Y, Lu H, Yang Z, Liu R, Wang J, Song X, Long J, Li Y, Lei D, Feng ZJournal:PubMed ID:24312503Striatal transplantation of dopaminergic (DA) neurons or neural stem cells (NSCs) has been reported to improve the symptoms of Parkinson's disease (PD), but the low rate of cell survival, differentiation, and integration in the host brain limits the therapeutic efficacy. We investigated the therapeutic effects of intracranial co-transplantation of mesencephalic ... MoreCyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.Authors:Akiyama H, Fukuda T, Tojima T, Nikolaev VO, Kamiguchi HJournal:J NeurosciPubMed ID:27194341'Graded distribution of intracellular second messengers, such as Ca(2+) and cyclic nucleotides, mediates directional cell migration, including axon navigational responses to extracellular guidance cues, in the developing nervous system. Elevated concentrations of cAMP or cGMP on one side of the neuronal growth cone induce its attractive or repulsive turning, respectively. ... MoreTLR7-mediated skin inflammation remotely triggers chemokine expression and leukocyte accumulation in the brain.Authors:McColl A, Thomson CA, Nerurkar L, Graham GJ, Cavanagh JJournal:J NeuroinflammationPubMed ID:27160148The relationship between the brain and the immune system has become increasingly topical as, although it is immune-specialised, the CNS is not free from the influences of the immune system. Recent data indicate that peripheral immune stimulation can significantly affect the CNS. But the mechanisms underpinning this relationship remain unclear. ... More