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Abstract
Staphylococcus aureus is a major human pathogen present on a third of the healthy population. The bacterium possesses an extensive arsenal of virulence factors. The pathogenicity is linked with S. aureus high plasticity and its exceptional ability to incorporate foreign genetic material. The aim of the present study was to perform molecular characterization of Staphylococcus aureus strains isolated from the clinical environment of the CHU-Z Abomey-Calavi/Sô-Ava. Isolation of Staphylococcus aureus bacterium was performed on Chapman agar. Toxin production by isolated S. aureus strains was investigated using the radial immunoprecipitation technique. A colorimetric assay was used to evaluate Staphylococcus aureus lipase (SA-Lipase) production. Finally, the expression of antibiotic resistance genes and genes encoding toxins production was investigated. Our data suggest that none of the isolated Staphylococcus aureus strains expressed the investigated toxin genes. Interestingly, SA-Lipase was produced by 14.28% of our isolated S. aureus strains. The mecA gene was present in 57.14% of the isolated strains, while PVL and TSST-1 genes were identified in 2.85 and 7.14% of S. aureus, respectively. Significant genetic diversity was observed along the hospital environment S. aureus strains. The present study reveals the level of virulence of S. aureus strains isolated in the different units of CHU-Z Abomey Calavi/Sô-Ava through the production of lipase, PVL, and epidermolysins. The molecular study has favored a genetic characterization within the isolated strains.
The authors declare that they have no conflicts of interest.
Figures
Figure 1
Photo showing the bands of…
Figure 1
Photo showing the bands of the 16S-23S rRNA gene. L: Molecular weight marker;…
Figure 1 Photo showing the bands of the 16S-23S rRNA gene. L: Molecular weight marker; S. aureus samples: (3, 8, 17, 27, 36).
Figure 2
Genetic diversity dendrogram as a…
Figure 2
Genetic diversity dendrogram as a function of the 16S-23S rRNA gene. The initials…
Figure 2 Genetic diversity dendrogram as a function of the 16S-23S rRNA gene. The initials indicate the service: N: neonatology; M: maternity; P: pediatrics; B: operating room; S: central sterilization.
Figure 3
Proportion of genes searched.
Figure 3
Proportion of genes searched.
Figure 3 Proportion of genes searched. See this image and copyright information in PMC
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