We have described a new method for monitoring of cell culture processes using green fluorescent protein (GFP) fluorescent intensity. GFP has been used as a ...
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We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pHi) in both ...
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We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pHi) in both gram- ...
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16 thg 12, 2014 · Abstract We have introduced two disulfide crosslinks into the loop regions on opposite ends of the beta barrel in superfolder green ...
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5 thg 9, 2018 · Thus, radiative rate in EGFP is circa two times lower, whereas non-radiative rate – 6 times lower than corresponding rates in BrUSLEE. \Phi ...
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21 thg 3, 2012 · Results represent a step forward in the study of pHi regulation and ... Green fluorescent protein (GFP) from Aequorea victoria and most of ...
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er; pHi, intracellular pH; GFP, green fluorescent protein; dsRNA, dou- ble stranded RNA; RNAi, double-stranded RNA mediated gene inter-.
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Here we have developed a Ca2+ probe based on a single GFP mol- ... that fluorescent changes responded not to pHi but to [Ca2+]i. In the.
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lular distribution of NHE3–GFP was obtained by confocal laser scanning microscopy (CLSM) ... SNARF-1 as an intracellular pH (pHi) indicator and confocal.
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The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Bị thiếu: phi | Phải bao gồm: phi
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required for this fluorescence, GFP expression can be used to monitor gene expression ... E. Southgate, J. N. Thomson, S. Brenner, Phi/los. Trans. R. Soc.
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27 thg 6, 2019 · single mutation must have only opened phi dynein partially. However, upon overexpression of. ∆C-HookA, GFP-dynein with nudAR1602 ...
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16 thg 2, 2007 · of 14-3-3-Phi/GFP. A transformed Arabidopsis line expressing the cod- ing region of the phi isoform coupled to GFP was used.
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20 thg 3, 2015 · Dnhe2 over-expression increases pHi in photoreceptor neurons. ... from other GFP-positive cells in three-dimensional tissue architecture.
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16 thg 4, 2022 · The fluorescence quantum yield ((\Phi\)) gives the efficiency of the ... Green fluorescence protein (GFP) is used in molecular biology to ...
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