Phua Kia Kien - INFORMM

Name

Phua Kia Kien

Current Position

Professor

Email

kkphua@usm.my

Office Telephone

+604-6534853/+604-6532628

Qualifications

PhD (Medical Immunology), University of Liverpool, 1985B.Sc. Hons (Med. Biochemistry), University of Liverpool, 1980

Research Interests

Immunology, Microbiology, Biotechnology, Information & Communication Technology, Medical Education

Scientific Research Overview

Pathogenesis of Salmonella Enterica Serovar Typhi

Recent projects in the lab include:

1. Molecular Analysis of Salmonella Enterica Serovar Typhi Isolates Using Pulsed-Field Gel Electrophoresis; Comparison Between Acute and Carriers of Typhoid Fever

2. A study of the role of Small RNAs in the Pathogenesis of Salmonella Enterica Serovar Typhi

3. Identification of Specific Genes in Salmonella Typhi, S. Paratyphi A, S. Paratyphi B, S. Paratyphi C, S. Enteritidis and S. Typhimurium, and Evaluation of the Antigenic Properties of Proteins Encoded by Putative S. Typhi Genes

Selected Publications

1. Mohd Redhuan, N.E., Chin, K.L., Adnan, A.S., Ismail, A., Balaram, P., Phua, K.K. Salivary anti-50 kDa antibodies as a useful biomarker for diagnosis of typhoid fever (2017) Journal of Clinical and Diagnostic Research, 11 (6), pp. DC10-DC11. DOI: 10.7860/JCDR/2017/21928.10055

2. Chin, K.L., Redhuan, N.E.M., Balaram, P., Phua, K.K., Ong, E.B.B. Detection of salivary IgA antibodies against the HlyE antigen as a diagnosis of typhoid fever (2016) Journal of Clinical and Diagnostic Research, 10 (6), pp. DM01-DM03.DOI: 10.7860/JCDR/2016/17801.7909

3. Ganjali Dashti, M., Abdeshahian, P., Sudesh, K., Phua, K.K. Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology (2016) Biofouling, 32 (4), pp. 477-487. DOI: 10.1080/08927014.2015.1135328

4. Hussain, A., Ong, E.B.B., Phei, P.S.C., Hossain, K., Balaram, P., Ismail, A., Kien, P.K. Role of tolc in virulence of salmonella enterica serovar typhi (2016) Journal of Pure and Applied Microbiology, 10 (2), pp. 887-896.

5. Goay, Y.X., Chin, K.L., Tan, C.L.L., Yeoh, C.Y., Ja'afar, J.N., Zaidah, A.R., Chinni, S.V., Phua, K.K. Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays (2016) BioMed Research International, 2016, art. no. 8905675, DOI: 10.1155/2016/8905675

6. Hussain, A., Boon Beng Ong, E., Phua, K.K., Balaram, P., Ismail, A. Construction and characterization of a Salmonella enterica serovar Typhi tolC deletion mutant (2014) Asian Pacific Journal of Tropical Disease, 4 (3), p. 226. DOI: 10.1016/S2222-1808(14)60517-X

7. Tan, C., Yuan Xin, G., Chiann Ying, Y., Ismail, A., Kia Kien, P. The identification and detection of a unique gene in Salmonella enterica subsp. enterica Weltevreden using PCR-based approach (2014) Asian Pacific Journal of Tropical Disease, 4 (3), p. 226.

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