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Download Free PDFProduction of Phytotoxic Metabolite Using Biphasic Fermentation System from Strain C1136 of Lasiodiplodia pseudotheobromae, a Potential Bioherbicidal AgentEmenike Irokanulo

Notulae Scientia Biologicae

https://doi.org/10.15835/NSB9310082visibility

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Abstract

Formulation of effective and environmental friendly bioherbicides depends on the type of fermentation medium used for the production of phytotoxic metabolites. The effect of biomass, colony forming unit and the phytotoxic metabolite produced from the biphasic fermentation was carried out, while the phytotoxic metabolite was  tested in vivo and in-vitro on Echinochola crus-galli and dicotyledonous Chromolaena odorata. The mutant strain of Lasiodiplodia pseudotheobromae C1136 (Lp90) produced the highest amount of conidia and the largest necrotic area on the two tested weeds when compared to its wild strain in the different biphasic media combinations. The study revealed that the biphasic system containing PDB + rice produced the highest bioherbicidal activities. Therefore, the phytotoxic metabolites from strain C1136 are suggested for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.

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References (29)

1. Adetunji CO, Oloke JK, Prasad GS, Adejumo IO (2017a). Effect of Lasiodiplodia pseudotheobromae isolates, a potential bioherbicide for Amaranthus hybridus L. in maize culture. Notulae Scientia Biologicae 9(1):131-137.
2. Adetunji C, Oloke J, Kumar A, Swaranjit S, Akpor B (2017b). Synergetic effect of rhamnolipid from Pseudomonas aeruginosa C1501 and phytotoxic metabolite from Lasiodiplodia pseudotheobromae C1136 on Amaranthus hybridus L. and Echinochloa crus-galli weeds. Environmental Science and Pollution Research 15(24):13700-13709.
3. Adetunji CO, Adejumo IO (2017). Nutritional assessment of mycomeat produced from different agricultural substrates using wild and mutant strains from Pleurotus sajor-caju during solid state Fermentation. Animal Feed Science and Technology 224:14-19.
4. Adetunji CO, Oloke JK (2013). Efficacy of freshly prepared pesta granular formulations from the multi-combination of wild and mutant strain of Lasiodiplodia pseudotheobromae and Pseudomonas aeruginosa. Albanian Journal of Agricultural Sciences 12(4):555-563.
5. Auld BA, Kim KU (1996). Weed management in rice (No. 139). Food and Agriculture Org.
6. Amusa NA (2005). Microbially produced phytotoxins and plant disease management. African Journal of Biotechnology 5:405-414.
7. Barranco-Florido JE, Alatorre-Rosas R, Gutierrez-Rojas M, Viniegra- Gonzalez G, Saucedo-Castaneda G (2002). Criteria for the selection of strains of entomopathogenic fungi Verticillium lecanii for solid state cultivation. Enzyme and Microbial Technology 30(7):910-915.
8. Boyetchko SM (1999). Innovative application of microbial agents for biological weed control. In: Mukerji KG, Chamola BP, Updahyay RK. (Eds). Biotechnological Approaches in Biocontrol of Plant Pathogens. Kluwer Academic/ Plenum, New York pp 73-97.
9. Derakhshan A, Rabindra RJ, Ramanujam B, Rahimi M (2008). Evaluation of different media and methods of cultivation on the production and viability of entomopathogenic fungi Verticillium lecanii (Zimm.) Viegas. Pakistian Journal of Biological Science 11(11):1506-1509.
10. Evidente A, Motta A (2001). Phytotoxins from fungi, pathogenic for agrarian, forestal and weedy plants. Bioactive Compound Natural Resources pp 473.
11. Francisco EA, Mochi DA, Correia ADCB, Monteiro AC (2006). Influence of culture media in viability test of conidia entomonopathogenic fungus. Ciencia Rural 36(4):1309-1312.
12. Guillon M (1997). Production of biopesticides: scale up and quality assurance. British Crop Production Council Symposium pp 151-162.
13. Rosas Acevedo JL, Alatorre Rosas R, Sampedro Rosas L, Valdez Carrasco J (1995). Sporulation of entomopathogenic fungi Hirsutella thompsonii Fisher and H. nodulosa petch in mixed culture. Revista Latinoamericana de Microbiologia 37:59-64.
14. Santoro PH, Neves PM, de Silva OJ, da Akimi RZ, Zorzetti J (2005). Spore production of Beauveria bassiana (Bals.) Vuill. in a two phase process using different liquid media. Semina: Agrarian Sciences 26:313-320.
15. SAS Institute-Statistical Analysis System (2001). Version 8.2 for Windows. Cary, NY: SAS Institute.
16. Schisler DA, Jackson MA, Bothast RJ (1991). Influence of nutrition during conidiation of Colletotrichum truncatum on conidial germination and efficacy in inciting disease in Seshania exaltata. Phytopathology 81(4):458-461.
17. Silman RW, Bothast RJ, Schisler DA (1993). Production of Colletotrichum truncatum for use as a mycoherbicide -effects of culture, drying and storage on recovery and efficacy. Biotechnology Advances 11(3):561- 575.
18. Strobel G, Kenfield D, Bunkers G, Sugawara F, Clardy J (1991). Phytotoxins as potential herbicides. In: Phytotoxins and their involvement in plant disease. Experientia 47:819-826.
19. Veloorvalappil NJ, Robinson BS, Prakasan P, Sreedharan S,Sailas B (2015). Biphasic fermentation is an efficient strategy for the overproduction of δ- Endotoxin from Bacillus thuringiensis. Applied Biochemistry and Biotechnology 175(3):1519-1535.
20. Vladimir G, Svetlana G, Jae SK (2014). Production of Beauveria bassiana air conidia by means of optimization of biphasic system technology. Brazilian Archives of Biology and Technology 57(4):571-577.
21. Hoagland RE, Boyette CD (1994). Pathogenic interactions of Alternaria crassaand phenolic metabolism in jimsonweed (Datura stramonium L.) varieties. Weed Science 42:44-49.
22. Jenkins NE, Goettel MS (1997). Methods for mass-production of microbial control agents for grasshoppers and locusts. The Memoirs of the Entomological Society of Canada 129(S171):37-48.
23. Jonsbu E, McIntyre M, Nielsen J (2002). The influence of carbon source and morphology on nystatin production by Streptomyces noursei. Journal of Biotechnology 95(2):133-144.
24. Ke W, Wei L, Jianrui S, Tao L (2015). Production, purification, and identification of cholest-4-en-3-one produced by cholesterol oxidase from Rhodococcus sp. in aqueous/organic biphasic system. Biochemistry Insights 8(S1):1-8.
25. Kaur P, Grewal HS, Kocher GS (2003). Production of α-amylase by Aspergillus niger using wheat bran in submerged and solid state fermentations. Indian Journal of Microbiology 43:143-145.
26. Kayode J, Ige OE (1998). A quantitative study of weed flora in three abandoned farmlands in Ekiti State. Biosciences Research Community 3:169-178.
27. Lomer CH, Lomer CJ (2008). Mass production of fungal pathogens for insect control: insect pathology manual. Retrieved 14 March 2017 from http://www.lubilosa.org.
28. Machado ACR, Monteiro AC, Mochi DA, Yoshida L (2009). Agroindustrial residues and byproducts and grains as substrates for the production of the entomopathogenic fungus Lecanicillium lecanii. Bragantia 68:483-491.
29. Penariol MC, Monteiro AC, Pitelli RA, Pereira GT (2008). Production of Bipolaris euphorbiae in solid and liquid culture media obtained from grains and agroindustrial residues. Bragantia 67:805-814.

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