Radioimmunoassay And Intramural Distribution Of PHI-IR In Human ...

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Abstract

The objective of this study was to develop a radioimmunoassay for PHI and use this to assess its intramural distribution in the human intestine. The antibody was harvested following immunization with porcine PHI conjugated to bovine serum albumin by glutaraldehyde, and the iodinated PHI tracer was prepared by the Iodo-gen method. The assay system showed no cross-reaction with other members of the glucagon-secretin family of peptides and was sensitive to changes of PHI of 2 fmol/tube (95% confidence). High concentrations of immunoreactive PHI were found in the human intestine, exclusively localized in the nonendocrine gut layers, suggesting a possible neuroendocrinological or neurotransmitter role for PHI.

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References

1. 1. FEBS Lett. 1980 Jun 2;114(2):240-2 - PubMed
2. 1. Gastroenterology. 1983 Apr;84(4):777-86 - PubMed
3. 1. Eur J Clin Invest. 1980 Feb;10(1):67-76 - PubMed
4. 1. Scand J Clin Lab Invest. 1972 Oct;30(2):221-32 - PubMed
5. 1. Life Sci. 1980 Nov 24;27(21):1947-51 - PubMed

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