Rapid Quantitative Detection Of li O157:H7 By A Impedance ...

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For Libraries For Publication Open Access Downloads About Us Contact Us Search Paper Titles Development of a New Signal Processing System for Pin-Cushion Position Sensitive Detector p.93 Finite Element Analysis on Multi Parameter Characteristics of Inductive Lubricating Oil Wear Debris Sensor p.97 Millimeter Wave Characterization of Wire Bond Transitions for W-Band Electromagnetic Sensor p.103 Optimizing Power Consumption for the Wireless Sensor Node p.107 Rapid Quantitative Detection of E.coli O157:H7 by a Impedance Immunosensor Based on Four-Wire Interdigitated Microelectrodes p.111 Reach and Application of Electronic Nose on Testing the Quality of Agriculture Products p.116 Research on Digital Substation IOT Based on SAW Sensing Technology p.125 Research on Pronunciation Assessment Detection Method Based on 3D Modeling and Sensor Technology p.129 Survey on Mobile Target Localization in Wireless Sensor Networks p.133 HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 738-739Rapid Quantitative Detection of E.coli O157:H7 by... Rapid Quantitative Detection of E.coli O157:H7 by a Impedance Immunosensor Based on Four-Wire Interdigitated Microelectrodes Article Preview

Abstract:

Rapid-response biosensing systems are necessary to counteract threats due to high-consequence pathogens. A disposable immunosensor suitable for quantitative determination of E.coli O157:H7 in pure cultures was investigated by electrochemical impedance spectroscopy (EIS). Electric responses of this biosensor for different cell concentrations at multi-frequencies were explored utilizing disposable screen-printed silver four-wire interdigitated microelectrodes (IMEs). Additionally, the best response frequency for the detection was studied, and the relationship between the impedance at this frequency and the concentrations of E.coli O157:H7 was established. The results showed that the impedance biosensor showed linearity from 1.15×103 CFU/mL to 1.15×106 CFU/mL at 100Hz, which yielded the model coefficient to 0.951.

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Periodical:

Applied Mechanics and Materials (Volumes 738-739)

Pages:

111-115

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.738-739.111 DOI link

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Online since:

March 2015

Authors:

Hai Yun Wu*, Xiao Ying Guo, Ya Ping Yu, Yue Ming Zuo, Yong Wei, Yuan Liu, Jin Xian Ma

Keywords:

E.coli O157:H7, Immunosensor, Impendance, Microelectrode, Quantitative Determination

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References

[1] J.A. Ho, H.W. Hsu, M.R. Huang: Anal. Biochem Vol. 330 (2004), pp.342-349.

Google Scholar

[2] Information on http: /www. ers. usda. gov/Data/FoodborneIllness/ecoli Intro. asp.

Google Scholar

[3] D.M. Brichta-Harhay, T.M. Arthur, J.M. Bosilevac, M.N. Guerini, N. Kalchayanand and M. Koohmaraie: J. Appl. Microbiol. Vol. 103 (2007), pp.1657-1668.

DOI: 10.1111/j.1365-2672.2007.03405.x

Google Scholar

[4] A. Jamshidi, M.R. Bassami and M. Rasooli: Iran J. Vet. Res. Vol. 9 (2008), pp.72-76.

Google Scholar

[5] J. Guan and R.E. Levin: Food Microbiol. Vol. 19 (2002), pp.159-165.

Google Scholar

[6] V.K. Sharma and Mol. Cell. Probe: Vol. 20 (2006), pp.298-306.

Google Scholar

[7] J.M. Simpson and D.V. Lim: Biosens. Bioelectron. Vol. 21 (2005), pp.881-887.

Google Scholar

[8] X. Zhou and X. Jiao: Food Control Vol. 16 (2005), pp.125-130.

Google Scholar

[9] Y.L. Bai W.C. Huang and S.T. Yang: Biotechnol. Bioeng. Vol. 98 (2007), pp.328-339.

Google Scholar

[10] C.J. Bell, D.A. Finlay, H.J. Clarke, M.J. Taylor and H.J. Ball: Vet. Microbiol. Vol. 85 (2002), pp.251-257.

Google Scholar

[11] Y. Bai: Lab. Med. Clin. Vol. 6 (2009), pp.1250-1252.

Google Scholar

[12] Z. Muhammad-Tahir and E.C. Alocilja: IEEE Sens. J. Vol. 3 (2003), pp.345-351.

Google Scholar

[13] Z. Muhammad-Tahir and E.C. Alocilja: Biosyst. Eng. Vol. 88 (2004), pp.145-151.

Google Scholar

[14] J.S. Yuk, J. Jin and E.C. Alocilja: Biosens. Bioelectron., Vol. 24 (2009), pp.1348-1352.

Google Scholar

[15] V. Madhukar and Y. Li: Biosens. Bioelectron. Vol. 22 (2007), pp.2408-2414.

Google Scholar

[16] V. Madhukar, Y. Li, S. Balaji and T. Steve: Sens. Actuators B Vol. 128 (2007), pp.99-107.

Google Scholar

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