Rapid Selection Of XO Embryonic Stem Cells Using Y Chromosome ...

Clipboard, Search History, and several other advanced features are temporarily unavailable. Skip to main page content

The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation Search: Search Advanced Clipboard Save Email Send to

• Clipboard
• My Bibliography
• Collections
• Citation manager

Display options Display options Format Abstract PubMed PMID

Save citation to file

Format: Summary (text) PubMed PMID Abstract (text) CSV Create file Cancel

Email citation

Subject: 1 selected item: 25008421 - PubMed To: From: Format: Summary Summary (text) Abstract Abstract (text) MeSH and other data Send email Cancel

Add to Collections

• Create a new collection
• Add to an existing collection

Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an error Please try again Add Cancel

Add to My Bibliography

• My Bibliography

Unable to load your delegates due to an error Please try again Add Cancel

Your saved search

Name of saved search: Search terms: Test search terms Would you like email updates of new search results? Saved Search Alert Radio Buttons

• Yes
• No

Email: (change) Frequency: Monthly Weekly Daily Which day? The first Sunday The first Monday The first Tuesday The first Wednesday The first Thursday The first Friday The first Saturday The first day The first weekday Which day? Sunday Monday Tuesday Wednesday Thursday Friday Saturday Report format: Summary Summary (text) Abstract Abstract (text) PubMed Send at most: 1 item 5 items 10 items 20 items 50 items 100 items 200 items Send even when there aren't any new results Optional text in email: Save Cancel

Create a file for external citation management software

Create file Cancel

Your RSS Feed

Name of RSS Feed: Number of items displayed: 5 10 15 20 50 100 Create RSS Cancel RSS Link Copy

Full text links

Springer Full text links

Actions

CiteCollectionsAdd to Collections

• Create a new collection
• Add to an existing collection

Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an errorPlease try again Add Cancel Display options Display options Format AbstractPubMedPMID

Share

• Permalink Copy

Page navigation

• Title & authors
• Abstract
• Similar articles
• Cited by
• References
• Publication types
• MeSH terms
• Substances
• Related information
• LinkOut - more resources

Title & authors Abstract Similar articles Cited by References Publication types MeSH terms Substances Related information LinkOut - more resources Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

We developed a transgenic mouse line with Y chromosome-linked green fluorescent protein expressing transgenes (Y-GFP) by the conventional microinjection into the pronucleus of C57BL/6J fertilized oocytes. Embryonic stem (ES) cells derived from Y-GFP mice enabled not only sexing but also the identification of 39, XO karyotype by the lack of Y chromosome. Actually, when fluorescence activated cell sorting (FACS) was applied to Y-GFP ES cells, non-fluorescent ES cells were conveniently collected and showed the lack of Y chromosome by PCR genotyping and Southern blot analysis. FACS analysis revealed Y chromosome loss occurred at 2.9 % of 40, XY ES cells after five passages. These Y-GFP ES cells are potentially applicable to reduce the time, cost and effort needed to generate the gene-targeted mice by the production of male and female mice derived from the same ES cell clone.

PubMed Disclaimer

Similar articles

• Identification, isolation and characterization of HCN4-positive pacemaking cells derived from murine embryonic stem cells during cardiac differentiation. Morikawa K, Bahrudin U, Miake J, Igawa O, Kurata Y, Nakayama Y, Shirayoshi Y, Hisatome I. Morikawa K, et al. Pacing Clin Electrophysiol. 2010 Mar;33(3):290-303. doi: 10.1111/j.1540-8159.2009.02614.x. Epub 2009 Nov 5. Pacing Clin Electrophysiol. 2010. PMID: 19895411
• Reestablishment of the inactive X chromosome to the ground state through cell fusion-induced reprogramming. Choi HW, Kim JS, Jang HJ, Choi S, Kim JH, Schöler HR, Do JT. Choi HW, et al. Cell Mol Life Sci. 2012 Dec;69(23):4067-77. doi: 10.1007/s00018-012-1139-6. Epub 2012 Nov 8. Cell Mol Life Sci. 2012. PMID: 26250157 Free PMC article.
• Donor hematopoietic cells from transgenic mice that express GFP are immunogenic in immunocompetent recipients. Bubnic SJ, Nagy A, Keating A. Bubnic SJ, et al. Hematology. 2005 Aug;10(4):289-95. doi: 10.1080/10245330500093468. Hematology. 2005. PMID: 16085541
• Rapid localization of transgenes in mouse chromosomes with a combined Spectral Karyotyping/FISH technique. Matsui S, Sait S, Jones CA, Nowak N, Gross KW. Matsui S, et al. Mamm Genome. 2002 Dec;13(12):680-5. doi: 10.1007/s00335-002-2197-0. Mamm Genome. 2002. PMID: 12514745
• Production of green fluorescent protein transgenic embryonic stem cells using the GENSAT bacterial artificial chromosome library. Tomishima MJ, Hadjantonakis AK, Gong S, Studer L. Tomishima MJ, et al. Stem Cells. 2007 Jan;25(1):39-45. doi: 10.1634/stemcells.2006-0173. Epub 2006 Sep 21. Stem Cells. 2007. PMID: 16990587 Free PMC article.

See all similar articles

Cited by

• Generation of functional oocytes from male mice in vitro. Murakami K, Hamazaki N, Hamada N, Nagamatsu G, Okamoto I, Ohta H, Nosaka Y, Ishikura Y, Kitajima TS, Semba Y, Kunisaki Y, Arai F, Akashi K, Saitou M, Kato K, Hayashi K. Murakami K, et al. Nature. 2023 Mar;615(7954):900-906. doi: 10.1038/s41586-023-05834-x. Epub 2023 Mar 15. Nature. 2023. PMID: 36922585
• Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage. Hirata W, Tomoda T, Yuri S, Isotani A. Hirata W, et al. Exp Anim. 2022 Feb 9;71(1):82-89. doi: 10.1538/expanim.21-0119. Epub 2021 Sep 20. Exp Anim. 2022. PMID: 34544911 Free PMC article.
• Identification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9. Zhao X, Wei W, Pan H, Nie J, Chen D, Zhang P, Chen F, Fu Q, Zuo E, Lu Y, Zhang M. Zhao X, et al. Sci Rep. 2019 Oct 4;9(1):14315. doi: 10.1038/s41598-019-50731-x. Sci Rep. 2019. PMID: 31586114 Free PMC article.
• Permissiveness to form pluripotent stem cells may be an evolutionarily derived characteristic in Mus musculus. Garbutt TA, Konneker TI, Konganti K, Hillhouse AE, Swift-Haire F, Jones A, Phelps D, Aylor DL, Threadgill DW. Garbutt TA, et al. Sci Rep. 2018 Oct 2;8(1):14706. doi: 10.1038/s41598-018-32116-8. Sci Rep. 2018. PMID: 30279419 Free PMC article.
• ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors. Skamagki M, Correia C, Yeung P, Baslan T, Beck S, Zhang C, Ross CA, Dang L, Liu Z, Giunta S, Chang TP, Wang J, Ananthanarayanan A, Bohndorf M, Bosbach B, Adjaye J, Funabiki H, Kim J, Lowe S, Collins JJ, Lu CW, Li H, Zhao R, Kim K. Skamagki M, et al. Nat Cell Biol. 2017 Sep;19(9):1037-1048. doi: 10.1038/ncb3598. Epub 2017 Aug 28. Nat Cell Biol. 2017. PMID: 28846095 Free PMC article.

References

1. 1. Nat Genet. 1998 Jul;19(3):220-2 - PubMed
2. 1. Gene. 1991 Dec 15;108(2):193-9 - PubMed
3. 1. Nat Methods. 2011 Oct 23;8(12):1071-7 - PubMed
4. 1. Nature. 2008 May 22;453(7194):519-23 - PubMed
5. 1. Nucleic Acids Res. 1987 Apr 10;15(7):2959-69 - PubMed

Show all 18 references

Publication types

• Research Support, Non-U.S. Gov't Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

MeSH terms

• Abnormal Karyotype Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Animals Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Blotting, Southern Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• DNA Primers / genetics Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Embryonic Stem Cells / cytology* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Female Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Flow Cytometry / methods* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Gene Transfer Techniques Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Genes, Y-Linked / genetics* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Green Fluorescent Proteins / genetics* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• In Situ Hybridization, Fluorescence Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Male Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Mice Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Mice, Inbred C57BL Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Mice, Transgenic Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Polymerase Chain Reaction Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Transgenes / genetics* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

Substances

• DNA Primers Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Green Fluorescent Proteins Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

Related information

• MedGen

LinkOut - more resources

• Full Text Sources

  • Springer

• Other Literature Sources

  • scite Smart Citations

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

Full text links [x] Springer [x] Cite Copy Download .nbib .nbib Format: AMA APA MLA NLM Send To

• Clipboard
• Email
• Save
• My Bibliography
• Collections
• Citation Manager

[x]

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.