Shim-pack Velox C18 - Shimadzu

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Shim-pack Velox C18

HPLC Column

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C18

Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.

• General purpose column for reversed-phase chromatography
• Highest hydrophobic retention among Shim-pack Velox series
• Compatible with moderately acidic to neutral mobile phases (pH 2-8)

Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment

The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

Column	L/dp	Flow rate (mL/min)	N	System suitability test result (Requirement: %RSD < 2.0 %)
VP-ODS (5 μm, 4.6 x 150 mm)	30,000	0.50	5,244	tR: 0.025 % Area: 0.175 % (n=6)
Velox C18 (2.7 μm, 4.6 x 100 mm)	37,037 (+23 %)	0.50	9,497 (+81 %)	tR: 0.035 % Area: 0.103 % (n=6)
		0.93	4,466 (-15 %)	tR: 0.084 % Area: 0.220 % (n=6)

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