The K/BxN Arthritis Model - PubMed

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Abstract

Mice expressing both the T cell receptor (TCR) transgene KRN and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes a similar arthritis in a wide range of mouse strains, due to autoantibodies recognizing glucose-6-phosphate isomerase (GPI). K/BxN transgenic mice have been useful for investigating the development of autoimmunity, and the serum transfer model has been particularly valuable in eliciting mechanisms by which anti-GPI autoantibodies induce joint-specific inflammation. This unit describes detailed methods for the maintenance of a K/BxN colony, induction of arthritis by serum transfer, clinical evaluation of arthritis, and measurement of anti-GPI antibodies.

Copyright 2008 by John Wiley & Sons, Inc.

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References

Literature Cited

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2. 1. Binstadt, B.A., Patel, P.R., Alencar, H., Nigrovic, P.A., Lee, D.M., Mahmood, U., Weissleder, R., Mathis, D., and Benoist, C. 2006. Particularities of the vasculature can promote the organ specificity of autoimmune attack. Nat. Immunol. 7: 284-292.
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