Thermo Scientific™ Pierce™ Hydroxylamine-HCl 25g Modification ...

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NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, including to cleave Asn-Gly peptide bonds and to deprotect (expose) sulfhydryls for conjugation following modification with SATA and similar reagents.

Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation. Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine-HCl.

Highlights:

  • Exposes protected sulfhydryl groups on SATA-modified molecules
  • Cleaves carbonyl-containing crosslinkers such as EGS

Reagent Properties:

  • Chemical name: Hydroxylamine Hydrochloride
  • Chemical formula: NH2OH·HCl
  • Molecular weight: 69.49
  • CAS Number: 5470-11-1
  • Form: White crystals, free of foreign material
  • Solubility: 10%
TRUSTED_SUSTAINABILITY

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