Triethylene Glycol Dimethacrylate Induces Large Deletions In The Hprt ...

Trang chủ » H 00164 » Triethylene Glycol Dimethacrylate Induces Large Deletions In The Hprt ...

Có thể bạn quan tâm

Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

Acrylate esters are applied in industrial and consumer products often associated with polymers and resins. The difunctional methacrylate, triethylene glycol dimethacrylate (TEGDMA), is also frequently included in dental composite materials. Recently, mutagenicity testing of the compound revealed the induction of gene mutations at the hprt locus in V79 cell [H. Schweikl, G. Schmalz, K. Rackebrandt, The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells, Mutat. Res. 415 (1998) 119-130]. In the present study, TEGDMA caused a dose dependent increase of the number of micronuclei in V79 cells. Furthermore, the mutation spectra induced in exon sequences of the hprt gene in HPRT-deficient V79 cell clones were analyzed by the polymerase chain reaction (PCR). No DNA sequence deletions were observed in spontaneously occurring HPRT-deficient cell clones at the molecular level after PCR analysis, indicating that all spontaneous mutations were caused by point mutations. However, TEGDMA treated V79 cell cultures exhibited different mutation spectra. Only one cell clone among a total of 25 contained all exon sequences of the hprt gene. Large DNA sequences were deleted in 24 cell clones. Partial gene deletions occurred in four clones from exon 5 through 9, and exon 1 was not amplified in one cell clone. Exon sequences of the hprt gene were totally deleted in 19 HPRT-deficient clones. The induction of mostly large deletions in the genome of mammalian cells, like the mutation spectra induced by TEGDMA in V79 cells here, is probably typical for crosslinking agents, including anticancer drugs. Identical types of mutations including chromosomal aberrations and the formation of micronuclei in vitro were observed for acrylates and methacrylates tested so far in various mutation assays. Therefore, we conclude by analogy that the induction of large DNA sequence deletions as shown here with the reactive dimethacrylate, triethylene glycol dimethacrylate, is probably common for acrylates and methacrylates.

PubMed Disclaimer

Publication types

  • Research Support, Non-U.S. Gov't Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

MeSH terms

  • Animals Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Cell Line Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Cricetinae Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Dose-Response Relationship, Drug Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Gene Deletion* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Hypoxanthine Phosphoribosyltransferase / drug effects Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Hypoxanthine Phosphoribosyltransferase / genetics* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Micronuclei, Chromosome-Defective / drug effects Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Micronucleus Tests Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Mutagens / toxicity* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Point Mutation / drug effects Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Polyethylene Glycols / toxicity* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Polymerase Chain Reaction Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Polymethacrylic Acids / toxicity* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

Substances

  • Mutagens Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Polymethacrylic Acids Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • triethylene glycol dimethacrylate Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Polyethylene Glycols Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Hypoxanthine Phosphoribosyltransferase Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

LinkOut - more resources

  • Full Text Sources

    • Elsevier Science

  • Other Literature Sources

    • The Lens - Patent Citations Database

  • Miscellaneous

    • NCI CPTAC Assay Portal

Từ khóa » H 00164