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TRPC5 Blocking Peptide (#BLP-CC020) is the original antigen used for immunization during Anti-TRPC5 Antibody (#ACC-020) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Certificate of AnalysisSDSApplications:wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes: 1. Anti-TRPC5Antibody (#ACC-020), (1:200). 2. Anti-TRPC5 Antibody, preincubated with TRPC5 Blocking Peptide (#BLP-CC020).
Expression of TRPC5 in rat cortexImmunohistochemical staining of rat cortex using Anti-TRPC5Antibody (#ACC-020). A. TRPC5 immunoreactivity (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Rat substantia nigra (De March, Z. et al. (2006) Neurosci. Lett. 402, 35.).
Properties
Sequence
(C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62).
Accession (Uniprot) NumberQ9UL62Peptide ConfirmationConfirmed by amino acid analysis and mass spectrometry.Purity>70%Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.Reconstitution100 µl double distilled water (DDW).Storage After Reconstitution-20°C.Antigen Preadsorption Control1 µg peptide per 1 µg antibody.Standard Quality Control Of Each LotWestern blot analysis.
Specific Use it with:
Anti-TRPC5 Antibody (#ACC-020) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPC5 from mouse, rat, and human samples.
Anti-TRPC5 Antibody (#ACC-020)
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