ANI Calculator

ANI Calculator

Welcome to CJ Bioscience's online Average Nucleotide Identity (ANI) calculator.

ANI has been widely used to compare two prokaryotic genome sequences when classifying and identifying bacteria by calculating the ANI value of two prokaryotic genome sequences. Our ANI Calculator uses the OrthoANIu algorithm, an improved iteration of the original OrthoANI algorithm, which uses USEARCH instead of BLAST (Lee et al. 2015). For large data sets, please refer to the standalone OrthoANIu tool available here.

Citing this software

Yoon, S. H., Ha, S. M., Lim, J. M., Kwon, S.J. & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek. 110:1281–1286.[Full Text]

• 1 Genomesequence A Upload FASTA

  Fasta QC

• 2 Genomesequence B Upload FASTA

  Fasta QC

• 3 CalculateANI Calculate

  OrthoANIu Results

  Calculation is taking a while. Please wait while we process your query.

Information

OK

Information

OK

Information

OK

Information

OK

Information

Reload Close

Info

Back Home

Session expired

Login

Warning

OK

Error

OK

Error

Reload Home DELETE Cancel OK Select Cancel OK

Information

OK

Upgrade your License

Upgrade license

Information

OK

Information

REQUEST

Academic/Non-profit Agreement (Annual Renewal Form)

Users affiliated with academic and non-profit institutions are entailed to free use of EzBioCloud's database and bioinformatics applications with conditions. Reproduction or redistribution of EzBioCloud is strictly prohibited by applicable law and regulations.

For the following cases, please contact [email protected] to obtain a license to use our services.

• Use in for-profit institutions or companies.
• Use for commercial projects by academic or non-profit organizations

Please login to the site to download the database. Click here to login or register if you do not have an account yet. By checking this box, I agree to only use EzBioCloud for non-profit purposes and to not reproduce or redistribute EzBioCloud in any form. Please click the checkbox to agree to the terms.

Your institution doesn't appear to be registered with us. Please request institution verification by clicking the button below.

The email domain associated with your EzBioCloud account doesn't appear to be an institution that we recognize. Please do not use gmail, hotmail etc. Use your institutional email.

If you believe this is an error, please contact [email protected] for further assistance.

Disagree

By not agreeing to only use EzBioCloud for non-profit purposes, you will not be granted free access to our database and bioinformatics applications on EzBioCloud.Please Confirm or Go back to change your status.

Confirm Go back

This browser is not supported. ×

We recommend to download the following browser from the link below.

Chrome

This browser is not supported.

We recommend to download the following browser from the link below.

Chrome

Hide this popup for the rest of today Ok