ANI Calculator - Kostas Lab

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§ ANI calculator

The ANI calculator estimates the average nucleotide identity using both best hits (one-way ANI) and reciprocal best hits (two-way ANI) between two genomic datasets, as calculated by Goris et al., 2007. Typically, the ANI values between genomes of the same species are above 95% (e.g., Escherichia coli). Values below 75% are not to be trusted, and AAI should be used instead. This tool supports both complete and draft genomes (multi-fasta). For an introductory comment, see Rodriguez-R & Konstantinidis, 2014. Examples: Escherichia coli, Escherichia, Escherichia vs Yersinia, Escherichia vs Xanthomonas.

§ Input data

User data (optional)

Your name, a valid e-mail address, and a name to identify the job.

Genome 1 or NCBI accession:

First genome in FastA format, or NCBI accession. For example, CM001142 for Escherichia fergusonii.

Genome 2 or NCBI accession:

Second genome in FastA format, or NCBI accession. For example, AE005174 for Escherichia coli.

§ ANI options

Alignment options Minimum length: bp Minimum identity: % Minimum alignments:

How alignments are filtered.

Fragment options Window size: bp Step size: bp

How to fragment the genomes.

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