Making Formalin-Fixed, Paraffin Embedded Blocks - PubMed

Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.

Keywords: Embedding; FFPE; Formalin; Paraffin; Paraffin blocks; Tissue orientation.

PubMed Disclaimer

Publication types

• Review Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

MeSH terms

• Fixatives / chemistry Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Formaldehyde / chemistry Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Humans Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Microtomy Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Nucleic Acids / genetics* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Paraffin Embedding / trends* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Proteomics* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Tissue Fixation / methods* Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

Substances

• Fixatives Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Nucleic Acids Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search
• Formaldehyde Actions
  • Search in PubMed
  • Search in MeSH
  • Add to Search

LinkOut - more resources

• Full Text Sources

  • Springer