Making LB Agar Plates - Bridges Lab Protocols

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SOP

500 mL makes about one sleeve of petri dishes
Add 25g LB Broth per Liter to an appropriately sized beaker
Add Water and Stir until clumps are gone
Add 15 g Agar per Liter , this will not dissolve
Transfer to a erlenmeyer flask at least 2x the volume that you are making
Autoclave for at least a 20 minute liquid cycle
Once the liquid is cool to the touch (dosent burn your hand to hold) add antibiotic and mix by swirling
Pour into plates, covering the surface and avoiding bubbles
Burn off bubbles with bunsen burner or pop with a sterile tip
Let plates cool with the lids ajar
Invert plates, place in sleeve and mark sleeve as LB/Amp or LB/Km depending on the antibiotic

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